Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | - |
Lactobacillus leichmannii | |
KBr | - |
Lactobacillus leichmannii | |
KCl | 0.1 M | Lactobacillus leichmannii | |
KI | - |
Lactobacillus leichmannii | |
MgCl2 | - |
Lactobacillus leichmannii | |
Urea | activates at 2 M about 1.5fold | Lactobacillus leichmannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxytubulosine | - |
Lactobacillus leichmannii | |
methotrexate | competitive | Lactobacillus leichmannii | |
p-chloromercuribenzoate | - |
Lactobacillus leichmannii | |
pegularinine | plant alkaloid | Lactobacillus leichmannii | |
tylophorinidine | plant alkaloid | Lactobacillus leichmannii | |
Urea | at concentration 3-4 M complete inactivation | Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
NADPH | - |
Lactobacillus leichmannii | |
0.0062 | - |
7,8-dihydrofolate | - |
Lactobacillus leichmannii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | - |
Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel filtration | Lactobacillus leichmannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
purified enzyme | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Lactobacillus leichmannii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, SDS-PAGE | Lactobacillus leichmannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Lactobacillus leichmannii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30% loss of activity after 60 min | Lactobacillus leichmannii |
45 | - |
90% loss of activity after 30 min | Lactobacillus leichmannii |
50 | - |
90% loss of activity after 5 min | Lactobacillus leichmannii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Lactobacillus leichmannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Lactobacillus leichmannii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000007 | - |
methotrexate | - |
Lactobacillus leichmannii | |
0.005 | - |
deoxytubulosine | - |
Lactobacillus leichmannii | |
0.007 | - |
tylophorinidine | - |
Lactobacillus leichmannii | |
0.009 | - |
pegularinine | - |
Lactobacillus leichmannii |