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Literature summary for 1.5.1.3 extracted from
- FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli (2003), J. Bacteriol., 185, 7015-7018.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Escherichia coli |  |
| additional information | no inhibition with trimethoprim up to 1.38 mM | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
NADPH | pH 6.0 | Escherichia coli | |
| 0.0095 | - |
7,8-dihydrofolate | pH 6.0 | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | cannot use NADH as reducing agent | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| dihydrobiopterin + NADPH | 10% of the activity with 7,8-dihydrofolate | Escherichia coli | ? + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FolM | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0059 | - |
methotrexate | competitive | Escherichia coli | |
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