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Literature summary for 1.5.1.3 extracted from
- Purification and kinetic properties of human recombinant dihydrofolate reductase produced in Bombyx mori chrysalides (2010), Appl. Biochem. Biotechnol., 162, 1834-1846.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a baculovirus system is established for the expression of this DHFR in Bombyx mori chrysalides. To isolate the expressed protein, whole infected pupae are homogenized | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| epicatechin gallate | - |
Homo sapiens |  |
| epigallocatechin gallate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
dihydrofolate | pH 7.5, 25°C | Homo sapiens | |
| 0.0074 | - |
NADPH | pH 7.5, 25°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00374 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrofolate + NADPH + H+ | - |
Homo sapiens | tetrahydrofolate + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHFR | - |
Homo sapiens |
| dihydrofolate reductase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51 | - |
NADPH | pH 7.5, 25°C, cosubstrate: dihydrofolate | Homo sapiens | |
| 51 | - |
dihydrofolate | pH 7.5, 25°C, cosubstrate: NADPH | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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