Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-bis-([N-(1-imino-1-guanidino-methyl)]sulfanylmethyl)-3,6-dimethyl-benzene | binding of the inhibitor has both a favorable entropy and enthalpy of binding. Positive binding cooperativity between inhibitor and the cofactor NADPH. Binding of inhibitor to DHFR is 285fold tighter in the presence of the NADPH cofactor than in its absence | Escherichia coli | |
1-([N-(1-imino-guanidino-methyl)]sulfanylmethyl)-3-trifluoromethyl-benzene | binding of the inhibitor has both a favorable entropy and enthalpy of binding. Positive binding cooperativity between inhibitor and the cofactor NADPH. Binding of inhibitor to DHFR in the absence of NADPH is not observed | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABQ4 | - |
- |
Purification (Comment) | Organism |
---|---|
using gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Escherichia coli |
dihydrofolate reductase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |