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Literature summary for 1.5.1.3 extracted from

  • Wang, W.; Gao, J.; Wang, J.; Liu, C.; Meng, Y.
    Cloning, expression and enzymatic properties analysis of dihydrofolate reductase gene from the silkworm, Bombyx mori (2012), Mol. Biol. Rep., 39, 10285-10291.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03825
-
7,8-dihydrofolate at pH 7.0 and 22°C Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
x * 21000, calculated from amino acid sequence Bombyx mori
25000
-
x * 25000, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydrofolate + NADPH + H+ Bombyx mori
-
5,6,7,8-tetrahydrofolate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori J7GQW2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bombyx mori
-
corpus allatum
-
Bombyx mori
-
fat body
-
Bombyx mori
-
malpighian tubule
-
Bombyx mori
-
midgut
-
Bombyx mori
-
silk gland
-
Bombyx mori
-
testis
-
Bombyx mori
-
trachea
-
Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH + H+
-
Bombyx mori 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Bombyx mori
? x * 21000, calculated from amino acid sequence Bombyx mori

Synonyms

Synonyms Comment Organism
DHFR
-
Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25
-
Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 when temperature is higher than 30°C, the enzyme activity is decreased rapidly but the comparatively lower velocity is maintained till 60°C Bombyx mori

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bombyx mori