Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03825 | - |
7,8-dihydrofolate | at pH 7.0 and 22°C | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, calculated from amino acid sequence | Bombyx mori |
25000 | - |
x * 25000, SDS-PAGE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Bombyx mori | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | J7GQW2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bombyx mori | - |
corpus allatum | - |
Bombyx mori | - |
fat body | - |
Bombyx mori | - |
malpighian tubule | - |
Bombyx mori | - |
midgut | - |
Bombyx mori | - |
silk gland | - |
Bombyx mori | - |
testis | - |
Bombyx mori | - |
trachea | - |
Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Bombyx mori | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Bombyx mori |
? | x * 21000, calculated from amino acid sequence | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | - |
Bombyx mori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | when temperature is higher than 30°C, the enzyme activity is decreased rapidly but the comparatively lower velocity is maintained till 60°C | Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bombyx mori |