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Literature summary for 1.5.1.3 extracted from

  • Khiralla, G.; El-Tarras, A.; Elhariry, H.
    Dihydrofolate reductase thermosensitive (ts-dfrA) mutant induces dihydrofolate overproduction by Lactobacillus plantarum (2019), Food Biotechnol., 33, 17-33 .
No PubMed abstract available

Application

Application Comment Organism
synthesis folate production by the mutant L62F in two-stage fermentation process with temperature shift-up from 30°C to 40°C increases by three-fold compared with the parental strain Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
L62F thermosensitive, mutant strain is to grow at 30°C but not at 40°C. Mutant protein shows a reduction in DHFR activity from 1.51 to 0.06 Units/mg protein, resulting in increasing DHF amount at the expense of THF. Total folate production by the mutant in two-stage fermentation process with temperature shift-up from 30°C to 40°C increases by three-fold compared with the parental strain Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum A0A0S4L1K0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
mutant L62F, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum
1.51
-
wild-type, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH + H+
-
Lactiplantibacillus plantarum 5,6,7,8-tetrahydrofolate + NADP+
-
?

Synonyms

Synonyms Comment Organism
DfrA
-
Lactiplantibacillus plantarum