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Literature summary for 1.5.1.30 extracted from

  • Chung, H.W.; Tu, S.C.
    Structure-function relationship of Vibrio harveyi NADPH-flavin oxidoreductase FRP: essential residues Lys167 and Arg15 for NADPH binding (2012), Biochemistry, 51, 4880-4887.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
K167A the mutant has apparently greatly increased Km and reduced kcat/Km for NADPH Vibrio harveyi
N134A the mutant shows increased Km and reduced kcat/Km for NADPH Vibrio harveyi
R133A the mutant shows increased Km and reduced kcat/Km for NADPH Vibrio harveyi
R15A the mutant has apparently greatly increased Km and reduced kcat/Km for NADPH Vibrio harveyi
R225A the mutant shows about wild type activity Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
NADPH wild type enzyme, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
0.01
-
NADPH mutant enzyme R225A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
0.016
-
NADPH mutant enzyme R133A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
0.034
-
NADPH mutant enzyme N134A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap DEAE column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH + H+
-
Vibrio harveyi FMNH2 + NADP+
-
?

Synonyms

Synonyms Comment Organism
FRP
-
Vibrio harveyi
NADPH-flavin oxidoreductase
-
Vibrio harveyi
NADPH-FMN oxidoreductase
-
Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
NADPH mutant enzyme N134A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
9.2
-
NADPH mutant enzyme R133A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
19.2
-
NADPH mutant enzyme R225A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
23.8
-
NADPH wild type enzyme, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Vibrio harveyi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
NADPH mutant enzyme R15A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
25
-
NADPH mutant enzyme K167A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
150
-
NADPH mutant enzyme N134A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
570
-
NADPH mutant enzyme R133A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
2000
-
NADPH mutant enzyme R225A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi
2670
-
NADPH wild type enzyme, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C Vibrio harveyi