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Literature summary for 1.5.1.33 extracted from

  • Robello, C.; Navarro, P.; Castanys, S.; Gamarro, F.
    A pteridine reductase gene ptr1 contiguous to a P-glycoprotein confers resistance to antifolates in Trypanosoma cruzi (1997), Mol. Biochem. Parasitol., 90, 525-535.
    View publication on PubMed
Search for more literature for 1.5.1.33

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, calculation from nucleotide sequence Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma cruzi the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates ?
-
 ?
additional information Trypanosoma cruzi Y the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
 Y strain
-
Trypanosoma cruzi Y
-
 Y strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH no activity with NADH and NADP+ Trypanosoma cruzi 5,6,7,8-Tetrahydrobiopterin + NADP+
-
 ?
Biopterin + NADPH no activity with NADH and NADP+ Trypanosoma cruzi Y 5,6,7,8-Tetrahydrobiopterin + NADP+
-
 ?
Dihydrobiopterin + NADPH
-
 Trypanosoma cruzi ?
-
 ?
Dihydrobiopterin + NADPH
-
 Trypanosoma cruzi Y ?
-
 ?
Dihydrofolate + NADPH
-
 Trypanosoma cruzi 5,6,7,8-Tetrahydrofolate + NADP+
-
 ?
Dihydrofolate + NADPH
-
 Trypanosoma cruzi Y 5,6,7,8-Tetrahydrofolate + NADP+
-
 ?
Dihydroneopterin + NADPH no activity Trypanosoma cruzi ?
-
 ?
dihydrosepiapterin + NADPH no activity Trypanosoma cruzi ?
-
 ?
folate + NADPH
-
 Trypanosoma cruzi 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
folate + NADPH
-
 Trypanosoma cruzi Y 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
additional information the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates Trypanosoma cruzi ?
-
 ?
additional information the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates Trypanosoma cruzi Y ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, calculation from nucleotide sequence Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 biopterin as substrate Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
4.7 7 significant decline of activity at pH 7 and 4.7 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Trypanosoma cruzi