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Literature summary for 1.5.1.36 extracted from

  • Hertzberger, R.; Arents, J.; Dekker, H.L.; Pridmore, R.D.; Gysler, C.; Kleerebezem, M.; de Mattos, M.J.
    H(2)O(2) production in species of the Lactobacillus acidophilus group a central role for a novel NADH-dependent flavin reductase (2014), Appl. Environ. Microbiol., 80, 2229-2239 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the NADH-dependent flavin reductase is encoded by two highly similar genes, LJ_0548 and LJ_0549, or ,nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme, DNA and amino acid sequence determination and analysis, a plasmid with the promoter region of the LJ_0045 lactate dehydrogenase gene and a bidirectional terminator is used for overexpression of the LJ_0548 and LJ_0549 genes Lactobacillus johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
FMN pH 7.0, 37°C Lactobacillus johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FAD + NADH + H+ Lactobacillus johnsonii
-
FADH2 + NAD+
-
r
FAD + NADH + H+ Lactobacillus johnsonii NCC 533
-
FADH2 + NAD+
-
r
FMN + NADH + H+ Lactobacillus johnsonii
-
FMNH2 + NAD+
-
r
FMN + NADH + H+ Lactobacillus johnsonii NCC 533
-
FMNH2 + NAD+
-
r
additional information Lactobacillus acidophilus no activity with NADPH ?
-
?
additional information Lactobacillus acidophilus NCC 533 no activity with NADPH ?
-
?
NADH + flavin Lactobacillus acidophilus
-
NAD+ + reduced flavin
-
?
NADH + flavin Lactobacillus acidophilus NCC 533
-
NAD+ + reduced flavin
-
?
riboflavin + NADH + H+ Lactobacillus johnsonii
-
reduced riboflavin + NAD+
-
r
riboflavin + NADH + H+ Lactobacillus johnsonii NCC 533
-
reduced riboflavin + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus Q74HL7 subunit 1
-
Lactobacillus acidophilus Q74HL8 subunit 2
-
Lactobacillus acidophilus NCC 533 Q74HL7 subunit 1
-
Lactobacillus acidophilus NCC 533 Q74HL8 subunit 2
-
Lactobacillus johnsonii Q74HL7 AND Q74HL8 genes LJ_0548 and LJ_0549, or nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme
-
Lactobacillus johnsonii NCC 533 Q74HL7 AND Q74HL8 genes LJ_0548 and LJ_0549, or nfr1 and nfr2, encoding the subunits 1 and 2 of the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q column chromatography, and Superdex 200 gel filtration Lactobacillus acidophilus
native enzyme from strain NCC 533 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Lactobacillus johnsonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
crude cell extract, about, with FAD, or FMN, or riboflavin, and NADH, pH 7.0, 37°C Lactobacillus johnsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+
-
Lactobacillus johnsonii FADH2 + NAD+
-
r
FAD + NADH + H+
-
Lactobacillus johnsonii NCC 533 FADH2 + NAD+
-
r
FMN + NADH + H+
-
Lactobacillus johnsonii FMNH2 + NAD+
-
r
FMN + NADH + H+
-
Lactobacillus johnsonii NCC 533 FMNH2 + NAD+
-
r
additional information no activity with NADPH Lactobacillus acidophilus ?
-
?
additional information the reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin, that give a similar activity, and is specific for NADH and not NADPH Lactobacillus johnsonii ?
-
?
additional information no activity with NADPH Lactobacillus acidophilus NCC 533 ?
-
?
additional information the reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin, that give a similar activity, and is specific for NADH and not NADPH Lactobacillus johnsonii NCC 533 ?
-
?
NADH + flavin
-
Lactobacillus acidophilus NAD+ + reduced flavin
-
?
NADH + flavin
-
Lactobacillus acidophilus NCC 533 NAD+ + reduced flavin
-
?
riboflavin + NADH + H+
-
Lactobacillus johnsonii reduced riboflavin + NAD+
-
r
riboflavin + NADH + H+
-
Lactobacillus johnsonii NCC 533 reduced riboflavin + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Lactobacillus acidophilus
dimer 2 * 20000, SDS-PAGE Lactobacillus johnsonii
More the two 20-kDa subunit proteins contain flavin mononucleotide (FMN) reductase conserved domains Lactobacillus johnsonii

Synonyms

Synonyms Comment Organism
flavin mononucleotide reductase
-
Lactobacillus johnsonii
FMN reductase
-
Lactobacillus johnsonii
LJ0548
-
Lactobacillus acidophilus
LJ0549
-
Lactobacillus acidophilus
LJ_0548
-
Lactobacillus johnsonii
LJ_0549
-
Lactobacillus johnsonii
NADH-dependent flavin reductase
-
Lactobacillus johnsonii
NADH-dependent flavin reductase
-
Lactobacillus acidophilus
nfr1
-
Lactobacillus johnsonii
nfr2
-
Lactobacillus johnsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Lactobacillus johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Lactobacillus johnsonii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Lactobacillus acidophilus
FMN
-
Lactobacillus acidophilus
NADH
-
Lactobacillus acidophilus
riboflavin
-
Lactobacillus acidophilus

General Information

General Information Comment Organism
malfunction deletion of the encoding genes genes nfr1 and nfr2 in Lactobacillus johnsonii leads to a 40fold reduction of hydrogen peroxide formation. H2O2 production in this mutant can only be restored by in trans complementation of both genes Lactobacillus johnsonii
physiological function the conserved NADH-dependent flavin reductase is prominently involved in H2O2 production in Lactobacillus johnsonii, overview Lactobacillus johnsonii