Cloned (Comment) | Organism |
---|---|
to enable functional assessment of the two-component monooxygenase system in Baeyer-Villiger oxidations, recombinant plasmids expressing Fred (FMN reductase (NADH)) in tandem with the respective 2,5-diketocamphane- and 3,6-diketocamphane 1,2-monooxygenase-encoding genes in Escherichia coli are constructed | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | competitive enzyme inhibitor | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
FMN | pH 7.5, 25°C | Pseudomonas putida | |
0.019 | - |
FAD | pH 7.5, 25°C | Pseudomonas putida | |
0.032 | - |
NADH | pH 7.5, 25°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | M4YFG7 | - |
- |
Pseudomonas putida ATCC 17453 | M4YFG7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, stable over several days | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | FMN and FAD are both substrates for the reductase. FMN is the favored substrate with a 2fold-higher rate constant and affinity that is about 5 times higher compared to that of FAD. With regard to electron donors, only NADH is effective whereas NADPH at a similar concentration acts as a very poor cosubstrate | Pseudomonas putida | FADH2 + NAD+ | - |
? | |
FAD + NADH + H+ | FMN and FAD are both substrates for the reductase. FMN is the favored substrate with a 2fold-higher rate constant and affinity that is about 5 times higher compared to that of FAD. With regard to electron donors, only NADH is effective whereas NADPH at a similar concentration acts as a very poor cosubstrate | Pseudomonas putida ATCC 17453 | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | FMN and FAD are both substrates for the reductase. FMN is the favored substrate with a 2fold-higher rate constant and affinity that is about 5 times higher compared to that of FAD. With regard to electron donors, only NADH is effective whereas NADPH at a similar concentration acts as a very poor cosubstrate | Pseudomonas putida | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | FMN and FAD are both substrates for the reductase. FMN is the favored substrate with a 2fold-higher rate constant and affinity that is about 5 times higher compared to that of FAD. With regard to electron donors, only NADH is effective whereas NADPH at a similar concentration acts as a very poor cosubstrate | Pseudomonas putida ATCC 17453 | FMNH2 + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 18000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
flavin reductase | - |
Pseudomonas putida |
Fred | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
the enzyme irreversibly unfolds with a half-life of about 80 min | Pseudomonas putida |
30 | 35 | the half-life of the enzyme is 5 to 20 min | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
128 | - |
FAD | pH 7.5, 25°C | Pseudomonas putida | |
283 | - |
FMN | pH 7.5, 25°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | in piperazine-HCl and phosphate buffer | Pseudomonas putida |
7.5 | - |
Tris/HCl buffer | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
NAD+ | pH 7.5, 25°C | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the degradation of (-)-camphor | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6700 | - |
FAD | pH 7.5, 25°C | Pseudomonas putida | |
7900 | - |
FMN | pH 7.5, 25°C | Pseudomonas putida |