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Literature summary for 1.5.1.38 extracted from

  • Chung, H.W.; Tu, S.C.
    Structure-function relationship of Vibrio harveyi NADPH-flavin oxidoreductase FRP: essential residues Lys167 and Arg15 for NADPH binding (2012), Biochemistry, 51, 4880-4887.
    View publication on PubMed
Search for more literature for 1.5.1.38

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
K167A the mutant has apparently greatly increased Km and severely reduced kcat/Km values for NADPH and leads to a slight increase in kcat/Km for NADH Vibrio harveyi
N134A the mutant shows strongly decreased kcat/Km for NADPH Vibrio harveyi
R133A the mutant shows strongly decreased kcat/Km for NADPH Vibrio harveyi
R15A the mutant has apparently greatly increased Km and severely reduced kcat/Km values for NADPH Vibrio harveyi
R225A the mutant shows decreased kcat/Km for NADPH Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 NADPH wild type enzyme, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
0.01
-
 NADPH mutant enzyme R225A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
0.016
-
 NADPH mutant enzyme R133A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
0.034
-
 NADPH mutant enzyme N134A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
FRP
-
 Vibrio harveyi
NADPH-flavin oxidoreductase
-
 Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
 NADPH mutant enzyme N134A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
9.2
-
 NADPH mutant enzyme R133A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
19.2
-
 NADPH mutant enzyme R225A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
23.8
-
 NADPH wild type enzyme, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
 NADPH mutant enzyme R15A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
25
-
 NADPH mutant enzyme K167A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
150
-
 NADPH mutant enzyme N134A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
570
-
 NADPH mutant enzyme R133A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
2000
-
 NADPH mutant enzyme R225A, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi
2670
-
 NADPH wild type enzyme, in 50 mM phosphate buffer, pH 7.0, at 22°C Vibrio harveyi