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Literature summary for 1.5.1.40 extracted from
- Function of coenzyme F420-dependent NADP reductase in methanogenic archaea containing an NADP-dependent alcohol dehydrogenase (1997), Arch. Microbiol., 168, 396-402.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
reduced coenzyme F420 | pH 8.0, 30°C | Methanogenium organophilum |  |
| 0.05 | - |
NADPH | pH 6.0, 30°C | Methanogenium organophilum | |
| 0.1 | - |
NADP+ | pH 8.0, 30°C | Methanogenium organophilum | |
| 0.1 | - |
oxidized coenzyme F420 | pH 6.0, 30°C | Methanogenium organophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Methanogenium organophilum |
| 90000 | - |
gradient gel electrophoresis under nondenaturating conditions | Methanogenium organophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacterium palustre | - |
- |
- |
| Methanobacterium palustre DSM 3108 | - |
- |
- |
| Methanoculleus thermophilus | - |
- |
- |
| Methanoculleus thermophilus DSM 3915 | - |
- |
- |
| Methanofollis liminatans | - |
- |
- |
| Methanofollis liminatans DSM 4140 | - |
- |
- |
| Methanogenium organophilum | P80951 | - |
- |
| Methanogenium organophilum DSM 3596 | P80951 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| stable under oxic conditions | Methanogenium organophilum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanogenium organophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
pH 8.0, 55°C, cell extract | Methanoculleus thermophilus |
| 0.06 | - |
pH 8.0, 37°C, cell extract | Methanofollis liminatans |
| 0.3 | - |
pH 8.0, 37°C, cell extract | Methanobacterium palustre |
| 15.1 | - |
pH 8.0, 30°C, cell extract | Methanogenium organophilum |
| 998 | - |
pH 8.0, 30°C, purified enzyme | Methanogenium organophilum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, enzyme solution is stable for several weeks | Methanogenium organophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | - |
Methanofollis liminatans DSM 4140 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | the enzyme exhibits a sequential kinetic mechanism | Methanogenium organophilum DSM 3596 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | - |
Methanobacterium palustre DSM 3108 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
| reduced coenzyme F420 + NADP+ | - |
Methanoculleus thermophilus DSM 3915 | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Methanogenium organophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F420-dependent NADP reductase | - |
Methanoculleus thermophilus |
| F420-dependent NADP reductase | - |
Methanofollis liminatans |
| F420-dependent NADP reductase | - |
Methanobacterium palustre |
| F420-dependent NADP reductase | - |
Methanogenium organophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | - |
- |
Methanogenium organophilum |
| 37 | - |
assay at | Methanofollis liminatans |
| 37 | - |
assay at | Methanobacterium palustre |
| 55 | - |
assay at | Methanoculleus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
pH optimum for reduction of oxidized coenzyme F420 with NADPH | Methanogenium organophilum |
| 9.2 | - |
pH optimum for reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | pH 7.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity, reduction of NADP+ with reduced coenzyme F420 | Methanogenium organophilum |
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Release 2023.1 (January 2023)
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