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Literature summary for 1.5.1.40 extracted from
- F420H2:NADP oxidoreductase from Methanobacterium thermoautotrophicum: identification of the encoding gene via functional overexpression in Escherichia coli (1998), FEBS Lett., 438, 124-126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Methanothermobacter thermautotrophicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method, 4°C | Methanothermobacter thermautotrophicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
NADPH | pH 6.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus |  |
| 0.07 | - |
NADP+ | pH 8.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
| 0.15 | - |
reduced coenzyme F420 | pH 8.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
| 0.3 | - |
oxidized coenzyme F420 | pH 6.0, temperature not specified in the publication | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
4 * 23500, calculated from sequence | Methanothermobacter thermautotrophicus |
| 28000 | - |
4 * 28000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 110000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | D9PVP5 | - |
- |
| Methanothermobacter thermautotrophicus DSM 2133 | D9PVP5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme at a protein concentration of 20 mg/ml is completely stable when stored at 4°C in 50 mM Tricine/KOH pH 8.0 containing 0.2 M KCl | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxidized coenzyme F420 + NADPH + H+ | - |
Methanothermobacter thermautotrophicus | reduced coenzyme F420 + NADP+ | - |
? | |
| oxidized coenzyme F420 + NADPH + H+ | - |
Methanothermobacter thermautotrophicus DSM 2133 | reduced coenzyme F420 + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 28000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| homotetramer | 4 * 23500, calculated from sequence | Methanothermobacter thermautotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F420H2:NADP oxidoreductase | - |
Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
reduction of oxidized coenzyme F420 with NADPH | Methanothermobacter thermautotrophicus |
| 8 | - |
reduction of NADP+ with reduced F420H2 | Methanothermobacter thermautotrophicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | calculated from sequence | - |
4.6 |
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