General Stability | Organism |
---|---|
neither high salt or protease inhibitors were effective in stabilizing the activity of the partially purified enzyme | Methanosphaera stadtmanae |
precipitation with (NH4)2SO4 reduces the stability of the enzyme | Methanosphaera stadtmanae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
reduced coenzyme F420 | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
0.0625 | - |
coenzyme F420 | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
0.142 | - |
NADPH | pH and temperature not specified in the publication | Methanosphaera stadtmanae | |
0.37 | - |
NADP+ | pH and temperature not specified in the publication | Methanosphaera stadtmanae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
50000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
60000 | - |
1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
148000 | - |
gel filtration, native PAGE | Methanosphaera stadtmanae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + NADP+ | Methanosphaera stadtmanae | - |
coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | Methanosphaera stadtmanae DSM 3091 | - |
coenzyme F420 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosphaera stadtmanae | - |
- |
- |
Methanosphaera stadtmanae DSM 3091 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosphaera stadtmanae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + NADP+ | - |
Methanosphaera stadtmanae | coenzyme F420 + NADPH + H+ | - |
r | |
reduced coenzyme F420 + NADP+ | - |
Methanosphaera stadtmanae DSM 3091 | coenzyme F420 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE | Methanosphaera stadtmanae |
Synonyms | Comment | Organism |
---|---|---|
NADP+:F420 oxidoreductase | - |
Methanosphaera stadtmanae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of coenzyme F420 | Methanosphaera stadtmanae |
8.5 | - |
reduction of NADP+ | Methanosphaera stadtmanae |