Literature summary for 1.5.1.40 extracted from

Joseph, E.; Le, C.Q.; Nguyen, T.; Oyugi, M.; Hossain, M.S.; Foss, F.W.; Johnson-Winters, K.
Evidence of negative cooperativity and half-site reactivity within an F420-dependent enzyme kinetic analysis of F420H2 NADP+ oxidoreductase (2016), Biochemistry, 55, 1082-1090 .

Search for more literature for 1.5.1.40

Cloned(Commentary)

Cloned (Comment)	Organism
cloning and recombinant expression in Escherichia coli strain C41(DE3)	Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the enzyme shows half-site reactivity and negative cooperativity (Koshland-Nemethy-Filmer model) in the reversible reduction of NADP+ through the transfer of a hydride from the reduced F420 cofactor, steady-state kinetic analysis revealing classical Michaelis-Menten kinetics with varying concentrations of the F420 redox moiety, and non-Michaelis-Menten kinetic behavior when NADPH is varied. Pre-steady-state, stopped flow, Single-turnover, and steady-state kinetics, detailed overview	Archaeoglobus fulgidus
4	-	oxidized coenzyme F420	pH 6.5, 22°C, recombinant enzyme	Archaeoglobus fulgidus
61.6	-	NADPH	pH 6.5, 22°C, recombinant enzyme	Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
reduced coenzyme F420 + NADP+	Archaeoglobus fulgidus	-	oxidized coenzyme F420 + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O29370	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from cell-free extracts of Escherichia coli strain C41(DE3) by heat treatment at 90°C for 30 min, ammonium sulfate fractionation with addition of 0.05% polyethylenimine, followed by an ion exchange chromatography, ultrafiltration, and gel filtration	Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
reduced coenzyme F420 + NADP+	-	Archaeoglobus fulgidus	oxidized coenzyme F420 + NADPH + H+	-	r

Synonyms

Synonyms	Comment	Organism
F420H2:NADP+ oxidoreductase	-	Archaeoglobus fulgidus
Fno	-	Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.27	-	oxidized coenzyme F420	pH 6.5, 22°C, recombinant enzyme	Archaeoglobus fulgidus
5.41	-	NADPH	pH 6.5, 22°C, recombinant enzyme	Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Archaeoglobus fulgidus

General Information

General Information	Comment	Organism
physiological function	half-site reactivity and negative cooperativity involving the important F420 cofactor-dependent enzyme. F420H2:NADP+ oxidoreductase (Fno), an F420 cofactor-dependent enzyme that catalyzes the reversible reduction of NADP+ through the transfer of a hydride from the reduced F420 cofactor. Fno may be a functional regulatory enzyme	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)