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Literature summary for 1.5.1.53 extracted from

  • Sobti, P.; Rothenberg, S.P.; Quadros, E.V.
    Radioenzymatic assay for reductive catalysis of N(5),N(10)-methylenetetrahydrofolate by methylenetetrahydrofolate reductase (2000), J. Biochem. Biophys. Methods, 46, 11-20 .
    View publication on PubMed
Search for more literature for 1.5.1.53

Application

Application Comment Organism
analysis sensitive radioenzymatic assay that measures directly the reductive catalysis of N ,N -methylenetetrahydrofolate. The radio-labeled substrate, [ C14]N ,N -methyl enetetrahydrofolate, is prepared by condensation of [C14]formaldehyde with tetrahydrofolate. Its stability is maintained for several months by storage at -80°C in a pH 9.5 buffer Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 5,10-methylenetetrahydrofolate pH 7.5, 23°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus D4A7E8 cf. EC 1.5.1.20
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH + H+
-
 Rattus norvegicus 5-methyltetrahydrofolate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
MTHFR
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus