Crystallization (Comment) | Organism |
---|---|
molecular docking of genistein. Gensitein may interfere with the binding site of NADH | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
genistein | 0.5 mM, almost complete inhibition. The inhibition is increased when genistein is preincubated with the enzyme and NADH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.175 | - |
NADH | pH 7.2, 37°C | Escherichia coli | |
0.24 | - |
NADH | presence of 0.05 mM genistein, pH 7.2, 37°C | Escherichia coli | |
0.25 | - |
NADH | presence of 0.1 mM genistein, pH 7.2, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEZ1 | cf. EC 1.5.1.20 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Escherichia coli | 5-methyltetrahydrofolate + NAD+ | - |
? |