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Literature summary for 1.5.1.6 extracted from

  • Cook, R.J.; Wagner, C.
    Enzymatic activities of rat liver cytosol 10-formyltetrahydrofolate dehydrogenase (1995), Arch. Biochem. Biophys., 321, 336-344.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 to 15 mM: 1.7fold increase in activity, higher concentrations have no further effect, it may reduce oxidized Cys-707 or another important sulfhydryl group Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
clone expressed in insect cells has both dehydrogenase and hydrolase activity and exhibits almost identical kinetic parameters to the native rat liver enzyme Rattus norvegicus
FDH is cloned Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C707A mutant with a complete loss of NADP+-dependent 10-formyltetrahydrofolate dehydrogenase activity, but no influence on hydrolase activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,10-dideazatetrahydrofolate 0.4 mM, 98% inhibition, folate analog Rattus norvegicus
additional information not inhibited by the folate analog N-[4-[[3-(2,4-diamino-1,6-dihydro-6-oxo-5-pyrimidinyl)propyl]amino]-benzoyl]-L-glutamate Rattus norvegicus
N-ethylmaleimide 1 mM or 10 mM, complete inhibition Rattus norvegicus
propanal FDH activity decreases with increasing concentrations, competes with 10-formyltetrahydrofolate for NADP+-dependent oxidation Rattus norvegicus
tetrahydrofolate
-
Oryctolagus cuniculus
tetrahydrofolate
-
Rattus norvegicus
Trypsin
-
Oryctolagus cuniculus
Trypsin tryptic digestion inactivates 10-formyltetrahydrofolate dehydrogenase but not hydrolase activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
NADP+
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99015
-
x * 99015, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
prone to oxidative inactivation, but may be partially reactivated by incubation with 100 mM 2-mercaptoethanol or 100 mM dithiothreitol Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ reaction mechanism Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-5,8-dideazafolate + NADP+ + H2O
-
Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
?
10-formyl-5,8-dideazafolate + NADP+ + H2O
-
Oryctolagus cuniculus 5,8-dideazafolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O
-
Oryctolagus cuniculus tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O mechanism which proceeds through thiohemiacetal and thioester intermediates, nucleophilic attack by the essential active site Cys-707 on the carbonyl group of 10-formyltetrahydrofolate, folate binding domain requires presence of an intact ring system Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Rattus norvegicus ?
-
?
additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Oryctolagus cuniculus ?
-
?
additional information enzyme catalyzes also NADP+-independent hydrolytic cleavage of 10-formyltetrahydrofolate to tetrahydrofolate and formate, hydrolysis is very inefficient compared to dehydrogenase reaction and may be an artifact of assay system Rattus norvegicus ?
-
?
additional information enzyme functions not as an aldehyde dehydrogenase in vivo, 10-formyltetrahydrofolate hydrolase activity is of no physiological significance, disulfiram may inhibit enzyme activity and probably perturb hepatic folate metabolism in vivo Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 99015, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
More identical with folate binding protein cytosol-I, FBP-CI Rattus norvegicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Rattus norvegicus
30
-
assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
10-formyltetrahydrofolate 10-formyltetrahydrofolate Oryctolagus cuniculus
folate 10-formyl-5,8-dideazafolate and 5,8-dideazafolate Rattus norvegicus
folate 10-formyl-5,8-dideazafolate and 5,8-dideazafolate Oryctolagus cuniculus
NADP+ NADP+-dependent Rattus norvegicus
NADP+ NADP+-dependent Oryctolagus cuniculus
NADP+ binding of substrate, 10-formyltetrahydrofolate or propanal, influences enzyme affinity for NADP+ Rattus norvegicus
tetrahydrofolate
-
Rattus norvegicus
tetrahydrofolate
-
Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.048
-
5,10-dideazatetrahydrofolate
-
Rattus norvegicus