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Literature summary for 1.5.1.6 extracted from

  • Min, H.; Shane, B.; Stokstad, E.L.R.
    Identification of 10-formyltetrahydrofolate dehydrogenase-hydrolase as a major folate binding protein in liver cytosol (1988), Biochim. Biophys. Acta, 967, 348-353.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
tetrahydrofolate
-
Rattus norvegicus
tetrahydrofolate
-
Sus scrofa
tetrahydropteroylhexaglutamate 1 mol per mol of tetrameric protein, potent product inhibitor: 0.0003 mM, 90% inhibition Sus scrofa
tetrahydropteroylpolyglutamate potent product inhibitor Rattus norvegicus
tetrahydropteroylpolyglutamate inhibition increases with increasing length of polyglutamyl chain; potent product inhibitor Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Sus scrofa
0.007
-
10-formyltetrahydrofolate
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE Sus scrofa
390000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O Sus scrofa disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male sprague-dawley rats
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.51
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
-
Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+ very tight binding of product, binds its product rather than its substrate ?
10-formyltetrahydrofolate + NADP+ + H2O
-
Sus scrofa tetrahydrofolate + CO2 + NADPH + H+ very tight binding of product, binds its product rather than its substrate ?
10-formyltetrahydrofolate + NADP+ + H2O disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydropteroylhexaglutamate + NADP+ + H2O
-
Sus scrofa tetrahydropteroylhexaglutamate + CO2 + NADPH
-
?
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O
-
Rattus norvegicus tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
?
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O affinity increases with increasing length of polyglutamyl chain Sus scrofa tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
?
additional information bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate Rattus norvegicus ?
-
?
additional information bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Rattus norvegicus
homotetramer 4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
More identical with folate binding protein cytosol-I, FBP-CI Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus
30
-
assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
NADP+
-
Rattus norvegicus
NADP+
-
Sus scrofa
tetrahydrofolate very tight binding Rattus norvegicus
tetrahydrofolate very tight binding Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
tetrahydrofolate
-
Sus scrofa
0.007
-
tetrahydrofolate
-
Rattus norvegicus