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Literature summary for extracted from

  • Donato, H.; Krupenko, N.I.; Tsybovsky, Y.; Krupenko, S.A.
    10-formyltetrahydrofolate dehydrogenase requires a 4-phosphopantetheine prosthetic group for catalysis (2007), J. Biol. Chem., 282, 34159-34166.
    View publication on PubMed


Cloned (Comment) Organism
expressed in Escherichia coli and Sf9 insect cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S354A no dehydrogenase activity Rattus norvegicus


Organism UniProt Comment Textmining
Rattus norvegicus

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information FDH requires posttranslational modification to become a functional enzyme (4'-phosphopantetheine prosthetic group) Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
5-fTHFSepharose column chromatography, Sephacryl S-300 gel filtration, and MonoQ column chromatography Rattus norvegicus


Reaction Comment Organism Reaction ID
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ FDH catalyzes the hydrolytic cleavage of the formyl group in the N-terminal catalytic domain, followed by NADP+-dependent oxidation of the formyl group toCO2 in the C-terminal aldehyde dehydrogenase domain Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+


Synonyms Comment Organism
10-formyltetrahydrofolate dehydrogenase
Rattus norvegicus
Rattus norvegicus
FDH the enzyme has also hydrolase and aldehyde dehydrogenase activities Rattus norvegicus


Cofactor Comment Organism Structure
NADP+ dependent Rattus norvegicus