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Literature summary for 1.5.1.6 extracted from

  • Strickland, K.C.; Krupenko, N.I.; Dubard, M.E.; Hu, C.J.; Tsybovsky, Y.; Krupenko, S.A.
    Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011), Chem. Biol. Interact., 191, 129-136.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
4'-phosphopantetheinyl transferase 4'-phosphopantetheinyl transferase activates dehydrogenase catalysis of ALDH1L2 by modifying Ser375 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S374A the mutant is activated by 4'-phosphopantetheinyl transferase Homo sapiens
S374A/S375A the mutant is not activated by 4'-phosphopantetheinyl transferase Homo sapiens
S375A the mutant is not activated by 4'-phosphopantetheinyl transferase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0114
-
10-formyltetrahydrofolate at pH 7.0 and 30Ā°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14500
-
4 * 14500, C-terminal domain, SDS-PAGE Homo sapiens
58000
-
C-terminal domain, gel filtration Homo sapiens
100000
-
full-length enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Homo sapiens
-
tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information S375 is the putative site of enzyme phosphopantetheinylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
-
Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 14500, C-terminal domain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
10-fTHF dehydrogenase
-
Homo sapiens
ALDH1L2
-
Homo sapiens
mtFDH mitochondrial isoform Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens