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Literature summary for 1.5.1.7 extracted from

  • Saunders, P.P.; Broquist, H.P.
    Saccharopine, an intermediate of the aminoadipic acid pathway of lysine biosynthesis. IV. Saccharopine dehydrogenase (1966), J. Biol. Chem., 241, 3435-3440.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol, 1 mM, stabilizes Saccharomyces cerevisiae
high salt concentration stabilizes Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate not Saccharomyces cerevisiae
p-hydroxymercuribenzoate NAD+ protects Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
2-oxoglutarate
-
Saccharomyces cerevisiae
0.46
-
NADH
-
Saccharomyces cerevisiae
12
-
L-lysine
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Saccharomyces cerevisiae involved in lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
?
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Saccharomyces cerevisiae last step of alpha-aminoadipate pathway for lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
baker's yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O high specificity with respect to coenzyme and substrate Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O i.e. saccharopine Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine biosynthesis Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
?
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O last step of alpha-aminoadipate pathway for lysine biosynthesis Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.8
-
NADH
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
L-lysine + 2-oxoglutarate + NADH Saccharomyces cerevisiae
10
-
or higher, saccharopine + NAD+ + H2O Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5.2 9 half-maximal activities at pH 5.2 and 9.0, L-lysine + 2-oxoglutarate + NADH Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae