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Literature summary for 1.5.1.7 extracted from

  • Hanke, R.; Hilgenberg, W.
    Charakterisierung der Saccharopin-Dehydrogenase aus Phycomyces blakesleeanus (1979), Z. Pflanzenphysiol., 92, 23-31.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
NADH
-
Phycomyces blakesleeanus
0.23
-
NADH 2-oxoglutarate Phycomyces blakesleeanus
1.34
-
L-lysine
-
Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Phycomyces blakesleeanus last step of alpha-aminoadipate pathway for lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Phycomyces blakesleeanus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.09
-
crude extract Phycomyces blakesleeanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Phycomyces blakesleeanus L-lysine + 2-oxoglutarate + NADH
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O last step of alpha-aminoadipate pathway for lysine biosynthesis Phycomyces blakesleeanus L-lysine + 2-oxoglutarate + NADH
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
increase of activity from 20┬░C to 40┬░C Phycomyces blakesleeanus

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
20 40 increase of activity from 20┬░C to 40┬░C Phycomyces blakesleeanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
L-lysine + 2-oxoglutarate + NADH Phycomyces blakesleeanus

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7.8 half-maximal activities at pH 5.6 and 7.8 Phycomyces blakesleeanus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Phycomyces blakesleeanus