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Literature summary for 1.5.1.7 extracted from
- Purification and characterization of saccharopine dehydrogenase from bakers yeast (1978), J. Biol. Chem., 253, 3666-3670.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes | Saccharomyces cerevisiae |
| unstable at enzyme concentration below 0.1 mg protein/ml | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | NAD+ and NADH protect | Saccharomyces cerevisiae |  |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | 40000 | gel filtration, sedimentation equilibrium centrifugation, SDS-PAGE | Saccharomyces cerevisiae |
| 39000 | - |
1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.09 | - |
crude extract | Saccharomyces cerevisiae |
| 106 | - |
after purification | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protein concentration above 0.1 mg/ml, pH 6.8, 1 mM EDTA, several months | Saccharomyces cerevisiae |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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