Any feedback?
Literature summary for 1.5.1.7 extracted from
- Saccharopine dehydrogenase (1971), Methods Enzymol., 17B, 124-129.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol, 1 mM, stabilizes | Saccharomyces cerevisiae |
| high salt concentration stabilizes | Saccharomyces cerevisiae |
| KCl, above 0.1 M stablizes | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | inhibition counteracted by 2-mercaptoethanol or glutathione | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
2-oxoglutarate | - |
Saccharomyces cerevisiae | |
| 0.46 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 12 | - |
L-lysine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
sucrose density gradient centrifugation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | high specificity with respect to coenzyme and substrate | Saccharomyces cerevisiae | L-lysine + 2-oxoglutarate + NADH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
L-lysine + 2-oxoglutarate + NADH | Saccharomyces cerevisiae |
| 10 | - |
or higher, saccharopine + NAD+ + H2O | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 5% of NADH reaction | Saccharomyces cerevisiae | |
| NAD+ | - |
Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
