Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-RIL cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C205S | the Km for N6-(L-1,3-dicarboxypropyl)-L-lysine decreases by more than 30fold for the C205S mutant | Saccharomyces cerevisiae |
C205V | the Km for N6-(L-1,3-dicarboxypropyl)-L-lysine decreases by 5fold for the C205V mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.019 | - |
NADH | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.05 | - |
NADH | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.11 | - |
2-oxoglutarate | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.11 | - |
2-oxoglutarate | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.2 | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.38 | - |
2-oxoglutarate | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.89 | - |
L-lysine | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
0.9 | - |
NAD+ | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.1 | - |
L-lysine | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.2 | - |
NAD+ | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.4 | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.8 | - |
NAD+ | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
3 | - |
L-lysine | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
6.7 | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41464 | - |
x * 41464, calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADH + H+ | Saccharomyces cerevisiae | - |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Saccharomyces cerevisiae | - |
L-lysine + 2-oxoglutarate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NiNTA affinity column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADH + H+ | - |
Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-lysine + 2-oxoglutarate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 41464, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
N6-(glutaryl-2)-L-lysine:NAD-oxidoreductase (L-lysine-forming) | - |
Saccharomyces cerevisiae |
SDH | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
NAD+ | wild type enzyme, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.4 | - |
NAD+ | mutant enzyme C205S, at pH 7.0 and 25°C | Saccharomyces cerevisiae | |
1.5 | - |
NAD+ | mutant enzyme C205V, at pH 7.0 and 25°C | Saccharomyces cerevisiae |