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Literature summary for 1.5.1.7 extracted from

  • Liu, J.; Li, Q.; Jiang, P.; Xu, Z.; Zhang, D.; Zhang, L.; Zhang, M.; Yu, H.; Song, C.; Tan, Q.; Shang, X.; Wang, R.
    Overexpression of the saccharopine dehydrogenase gene improves lysine biosynthesis in Flammulina velutipes (2019), J. Basic Microbiol., 59, 890-900 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Fvsdh, cloned from genomic DNA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree Flammulina velutipes

Protein Variants

Protein Variants Comment Organism
additional information enzyme overexpression from endogenous gene Fvsdh resulting in 1.1-3.0fold increased enzyme content in randomly selected transgenic strains, lysine contents are also increased from 1.12 to 1.3fold in these five transformants, except for strain T3, in which the lysine contents are the same as the control. Effects on other genes in SDH overexpressing cells: expression of the two genes, AAT and AAR, is increased in all five transformants compared with that in the wild-type. The expression of the HCS gene is increased in four transformants except for T5. The expression of the HAH gene is increased in T1, T3, and T5 but decreased in T2 and is almost the same as that of the wild-type in T4. The expression of the HIDH gene is increased in four transformants except for T4. The expression of the SDR gene is increased in four transformants except for T5, overview Flammulina velutipes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Flammulina velutipes
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L-lysine + 2-oxoglutarate + NADH + H+
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Flammulina velutipes Dan3
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L-lysine + 2-oxoglutarate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Flammulina velutipes A0A4Y5PV56
-
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Flammulina velutipes Dan3 A0A4Y5PV56
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-

Source Tissue

Source Tissue Comment Organism Textmining
additional information tissue expression analysis using primordium, elongation-pileus, elongation-stipe, maturation-pileus, and maturation-stipe from three developmental stages reveals differential expression of sdh during the different developmental stages of Flammulina velutipes. The expression level of gene Fvsdh is higher in the pileus than in the stipe whether in the elongation or maturation stage. The lysine contents are 3.03 and 2.95 mg/g in maturation-pileus and elongation-pileus, respectively. In the elongation-stipe and maturation-stipe, respectively, lysine contents are 2.49 and 2.07 mg/g Flammulina velutipes
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mycelium
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Flammulina velutipes
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pileus
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Flammulina velutipes
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stipe
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Flammulina velutipes
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Flammulina velutipes L-lysine + 2-oxoglutarate + NADH + H+
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Flammulina velutipes Dan3 L-lysine + 2-oxoglutarate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
Fvsdh
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Flammulina velutipes
saccharopine dehydrogenase
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Flammulina velutipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Flammulina velutipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Flammulina velutipes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Flammulina velutipes
NADH
-
Flammulina velutipes

General Information

General Information Comment Organism
metabolism saccharopine dehydrogenase regulates the last step of fungal lysine biosynthesis Flammulina velutipes