Cloned (Comment) | Organism |
---|---|
gene Fvsdh, cloned from genomic DNA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree | Flammulina velutipes |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme overexpression from endogenous gene Fvsdh resulting in 1.1-3.0fold increased enzyme content in randomly selected transgenic strains, lysine contents are also increased from 1.12 to 1.3fold in these five transformants, except for strain T3, in which the lysine contents are the same as the control. Effects on other genes in SDH overexpressing cells: expression of the two genes, AAT and AAR, is increased in all five transformants compared with that in the wild-type. The expression of the HCS gene is increased in four transformants except for T5. The expression of the HAH gene is increased in T1, T3, and T5 but decreased in T2 and is almost the same as that of the wild-type in T4. The expression of the HIDH gene is increased in four transformants except for T4. The expression of the SDR gene is increased in four transformants except for T5, overview | Flammulina velutipes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Flammulina velutipes | - |
L-lysine + 2-oxoglutarate + NADH + H+ | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Flammulina velutipes Dan3 | - |
L-lysine + 2-oxoglutarate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flammulina velutipes | A0A4Y5PV56 | - |
- |
Flammulina velutipes Dan3 | A0A4Y5PV56 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | tissue expression analysis using primordium, elongation-pileus, elongation-stipe, maturation-pileus, and maturation-stipe from three developmental stages reveals differential expression of sdh during the different developmental stages of Flammulina velutipes. The expression level of gene Fvsdh is higher in the pileus than in the stipe whether in the elongation or maturation stage. The lysine contents are 3.03 and 2.95 mg/g in maturation-pileus and elongation-pileus, respectively. In the elongation-stipe and maturation-stipe, respectively, lysine contents are 2.49 and 2.07 mg/g | Flammulina velutipes | - |
mycelium | - |
Flammulina velutipes | - |
pileus | - |
Flammulina velutipes | - |
stipe | - |
Flammulina velutipes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Flammulina velutipes | L-lysine + 2-oxoglutarate + NADH + H+ | - |
r | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Flammulina velutipes Dan3 | L-lysine + 2-oxoglutarate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Fvsdh | - |
Flammulina velutipes |
saccharopine dehydrogenase | - |
Flammulina velutipes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Flammulina velutipes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Flammulina velutipes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Flammulina velutipes | |
NADH | - |
Flammulina velutipes |
General Information | Comment | Organism |
---|---|---|
metabolism | saccharopine dehydrogenase regulates the last step of fungal lysine biosynthesis | Flammulina velutipes |