Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | L-lysine formation is not activated by (NH4)2SO4 | Homo sapiens |
General Stability | Organism |
---|---|
EDTA and 2-mercaptoethanol stabilize during purification | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
detergent | e.g. Aerosol 22, Ultrawet 60L, deoxycholic acid, cetyltrimethyl ammonium bromide, complete inactivation | Homo sapiens | |
L-Lysine-p-nitroanilide | inhibition noncompetitive with L-lysine, competitive with 2-oxoglutarate | Homo sapiens | |
additional information | not inhibited by the detergents Lubrol PX, Tween 40, saccharopine formation is not inhibited by (NH4)2SO4 | Homo sapiens | |
S-2-aminoethyl-L-cysteine | - |
Homo sapiens | |
saccharopine | 1 mM, 30% inhibition, product inhibition; competitive with 2-oxoglutarate, noncompetitive with L-lysine, uncompetitive with NADPH | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
480000 | - |
gel filtration, no separation of L-lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
450fold purification, no separation of lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ | reaction mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, pH 7.5, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, storage conditions which are most effective in preserving enzyme activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | reverse reaction not detected | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | reverse reaction not detected | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
4°C, in presence of 5 mM 2-mercaptoethanol and 0.1 mM EDTA, highest stability | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for NADPH | Homo sapiens | |
NADPH | NADPH required as cofactor | Homo sapiens |