Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, slight activation, effect not consistant | Homo sapiens | |
Triton X-100 | 1%, slight activation, effect not consistant | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | hyperlysinemia: genetic disease, deficiency of lysine-ketoglutarate reductase results in extreme elevations of serum lysine | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 3 mM, 28% loss of activity | Homo sapiens | |
cadaverine | 3 mM, 26% loss of activity | Homo sapiens | |
Carbamoylphosphate | 3 mM, 25% loss of activity | Homo sapiens | |
CoCl2 | 1 mM, 82% loss of activity | Homo sapiens | |
CuSO4 | 1 mM, 87% loss of activity | Homo sapiens | |
DL-pipecolic acid | 6 mM, 47% loss of activity | Homo sapiens | |
HgCl2 | 0.01 mM, 89% loss of activity | Homo sapiens | |
hydroxylamine | 3 mM, 33% loss of activity | Homo sapiens | |
L-Glutamic acid | 3 mM, 40% loss of activity | Homo sapiens | |
L-Homocitrulline | 3 mM, 49% loss of activity | Homo sapiens | |
L-Lysylglycine | 3 mM, 25% loss of activity | Homo sapiens | |
L-ornithine | 3 mM, 39% loss of activity | Homo sapiens | |
leucine | - |
Homo sapiens | |
MgSO4 | 1 mM, 79% loss of activity | Homo sapiens | |
MnCl2 | 1 mM, 52% loss of activity | Homo sapiens | |
additional information | not inhibited by 40 mM ammonium chloride, inhibition by NH4+ is probably due to contamination with glutamic dehydrogenase | Homo sapiens | |
saccharopine | 1 mM, 30% inhibition, product inhibition | Homo sapiens | |
ZnCl2 | 3 mM, complete inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADPH | - |
Homo sapiens | |
1 | - |
2-oxoglutarate | - |
Homo sapiens | |
1.5 | - |
L-lysine | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Homo sapiens | saccharopine pathway is the major route of lysine breakdown | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
44.6fold partial purification from liver | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | after liver next highest enzyme concentration | Homo sapiens | - |
kidney | low enzyme concentration | Homo sapiens | - |
liver | highest enzyme concentration | Homo sapiens | - |
additional information | trace enzyme activity in muscle, brain, and spleen, no activity in pancreas, adrenal, lung, gut, leucocytes, erythrocytes | Homo sapiens | - |
skin | low enzyme concentration | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 1 month, 50% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-delta-hydroxylysine + 2-oxoglutarate + NADPH | 18% of velocity with equimolar concentration of L-lysine | Homo sapiens | ? | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | reverse reaction not detected | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ir | |
L-lysine + 2-oxoglutarate + NADPH | saccharopine pathway is the major route of lysine breakdown | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
additional information | less than 2% of the rate of L-lysine: D-lysine, cadaverine, delta-aminovaleric acid, alpha-acetyl-L-lysine, alpha-amino-epsilon-hydroxycaproic acid, L-lysylglycine, L-2,4-diaminobutyric acid, DL-pipecolic acid, L-ornithine, epsilon-aminocaproic acid, 4% of the rate of L-lysine: DL-alpha,epsilon-diaminopimelic acid, 7% of the rate of 2-oxoglutarate: oxaloacetic acid, less than 2% of the rate of 2-oxoglutarate: 2-oxoadipate, pyruvate, 2-oxoisocaproate, 2-oxoisovalerate, levulinate, 2-hydroxyglutarate | Homo sapiens | ? | - |
? | |
S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH | 47% of velocity with equimolar concentration of L-lysine | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, unstable above | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.8 | about 50% of activity maximum at pH 7.2 and 8.8 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
10 min, 50% loss of activity | Homo sapiens |
4.6 | 4.9 | 10 min, stable | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |