General Stability | Organism |
---|---|
presence of 5% w/v insoluble polyvinylpyrrolidone and 10% v/v glycerol in extraction buffer and glycerol in running buffers is crucial to maintain enzyme activity | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
saccharopine | product inhibition, competitive with 2-oxoglutarate, noncompetitive with L-lysine | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADPH | - |
Oryza sativa | |
0.79 | - |
2-oxoglutarate | - |
Oryza sativa | |
4.48 | - |
L-lysine | - |
Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
202000 | - |
non-denaturing PAGE, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, one band represents the holoenzyme and shows higher enzyme activities compared to a second band of 396 kDa with much lower enzyme activities indicating a multimeric structure | Oryza sativa |
203000 | - |
gel filtration, bifunctional protein containing lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Oryza sativa | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Oryza sativa IAC 165 | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | Oryza sativa IAC 165 | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
IAC 165 | - |
Oryza sativa IAC 165 | - |
IAC 165 | - |
Purification (Comment) | Organism |
---|---|
38fold purification | Oryza sativa |
partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ | ordered sequence mechanism, where 2-oxoglutarate is first substrate and saccharopine is last product | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | developing | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Oryza sativa | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | regulation of lysine catabolism may be essential for lysine accumulation in rice plants | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Oryza sativa IAC 165 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa | ? | - |
? | |
additional information | bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities | Oryza sativa IAC 165 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 369 kDa band may represent a dimeric or tetrameric molecule, multimeric form results from the association of the 202 kDa LOR/SDH band | Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
saccharopine | - |
Oryza sativa |