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Literature summary for 1.5.1.8 extracted from

  • Epelbaum, S.; McDevitt, R.; Falco, S.C.
    Lysine-ketoglutarate reductase and saccharopine dehydrogenase from Arabidopsis thaliana: Nucleotide sequence and characterization (1997), Plant Mol. Biol., 35, 735-748.
    View publication on PubMed
Search for more literature for 1.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
isolation and cloning of the cDNA and single copy gene encoding bifunctional enzyme with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9, no expression in Escherichia coli, gene product with amino domain corresponding to LKR and carboxy domain corresponding to SDH Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol lack of targeting sequence in LKR-SDH gene implies localization in cytosol Arabidopsis thaliana 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Arabidopsis thaliana
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 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Nicotiana tabacum enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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 ecotype Landsberg erecta and Columbia
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Nicotiana tabacum
-
-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH
-
 Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Nicotiana tabacum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Arabidopsis thaliana N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation Nicotiana tabacum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 117000, bifunctional protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities, calculated from cDNA sequence Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Nicotiana tabacum
NADPH
-
 Arabidopsis thaliana