Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in apoform or in complex with substrates, products, and/or NADP+, hanging drop vapor diffusion method, mixing of 0.0015 ml of a 1.2 mg/ml protein solution with 0.0015 ml of well solution containing 100 mM Bis-Tris, pH 5.45, 200 mM ammonium acetate, and 24% PEG 3350, with NADP+, or 270-290 mM ammonium formate and 28% PEG 3350, with pseudopaline, 4°C, X-ray diffraction structure determination and analysis at 1.57-2.18 A resolution, molecular replacement and modelling | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics. The reverse reaction initiated with (R)-pseudopaline shows steady-state turnover with a kcat value about 60% slower than the previously published forward rates | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa ATCC 15692 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa 1C | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa PRS 101 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa DSM 22644 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa CIP 104116 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa LMG 12228 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | Pseudomonas aeruginosa JCM 14847 | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | pseudopaline + NADP+ + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HUX5 | - |
- |
Pseudomonas aeruginosa 1C | Q9HUX5 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9HUX5 | - |
- |
Pseudomonas aeruginosa CIP 104116 | Q9HUX5 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HUX5 | - |
- |
Pseudomonas aeruginosa JCM 14847 | Q9HUX5 | - |
- |
Pseudomonas aeruginosa LMG 12228 | Q9HUX5 | - |
- |
Pseudomonas aeruginosa PRS 101 | Q9HUX5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa ATCC 15692 | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa 1C | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa PRS 101 | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa DSM 22644 | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa CIP 104116 | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa LMG 12228 | pseudopaline + NADP+ + H2O | - |
r | |
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ | i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine | Pseudomonas aeruginosa JCM 14847 | pseudopaline + NADP+ + H2O | - |
r | |
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa ATCC 15692 | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa 1C | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa PRS 101 | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa CIP 104116 | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa LMG 12228 | ? | - |
- |
|
additional information | full knowledge of opine metallophore stereochemistry is important as it is likely to influence receptor recognition as well as the coordination geometry for metal complexes. This is especially important for pseudopaline, because it incorporates an extra carboxylate ligand fromx022-oxoglutarate. As both PaODH and SaODH belong to the (R)-opine producing structural class, it is oproposed that they produce (R)-opine metallophores. The opine dehydrogenase reaction is reversible only for the opine metallophore product with (R)-stereochemistry at carbon C2 of the alpha-keto acid (prochiral prior to catalysis). Kinetic analysis using stopped-flow spectrometry with (R)- or (S)-staphylopine and kinetic and structural analysis with (R)- and (S)-pseudopaline confirms catalysis in the reverse direction for only (R)-staphylopine and (R)-pseudopaline, verifying the stereochemistry of these two opine metallophores. No NADP+ reduction with (S)-pseudopaline. Structural analysis at 1.57-1.85 A resolution captures the hydrolysis of (R)-pseudopaline and allows identification of a binding pocket for the L-histidine moiety of pseudopaline formed through a repositioning of Phe340 and Tyr289 during the catalytic cycle. Transient-state kinetic analysis reveals an ordered release of NADP+ followed by staphylopine, with staphylopine release being the rate-limiting step in catalysis. PaODH binds (S)-pseudopaline in a noncatalytic complex, (S)-pseudopaline binds above the nicotinamide ring of NADP+, structure analysis of enzyme-bound substrates, and reaction mechanism, detailed overview. PaODH crystals catalyze the hydrolysis of (R)-pseudopaline | Pseudomonas aeruginosa JCM 14847 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
cntM | - |
Pseudomonas aeruginosa |
ODH | - |
Pseudomonas aeruginosa |
PA4835 | - |
Pseudomonas aeruginosa |
PaODH | - |
Pseudomonas aeruginosa |
pseudopaline synthase | UniProt | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | PaODH catalyzes a reversible reaction that specifically produces the (R)-opine metallophore diastereomer, kinetic mechanism, overview | Pseudomonas aeruginosa |