Cloned (Comment) | Organism |
---|---|
native and mutant enzyme | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
R49K mutant after reconstitution with FAD, sitting drop vapor diffusion method, high salt or high PEG conditions | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
R49K | 40fold decrease of turnover, 150fold decrease of catalytic efficiency, structure very similar to wild type enzyme | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylthioacetate | - |
Bacillus sp. (in: Bacteria) | |
N-(cyclopropyl)glycin | suicide substrate | Bacillus sp. (in: Bacteria) | |
pyrrole-2-carboxylate | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
0.29 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
7.6 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
28.8 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + O2 + H2O | Bacillus sp. (in: Bacteria) | - |
formaldehyde + glycine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | P40859 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant native and mutant enzyme | Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 | mechanism suggested | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine | Bacillus sp. (in: Bacteria) | ? | - |
? | |
sarcosine + O2 + H2O | - |
Bacillus sp. (in: Bacteria) | formaldehyde + glycine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monomeric sarcosine oxidase | - |
Bacillus sp. (in: Bacteria) |
MSOX | - |
Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
sarcosine | R49K mutant, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
101 | - |
sarcosine | native enzyme, pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Bacillus sp. (in: Bacteria) |