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Literature summary for 1.5.3.1 extracted from

  • Pundir, C.; Chauhan, N.; Kumari, G.; Vandana, C.
    Immobilization of Arthrobacter sarcosine oxidase onto alkylamine and arylamine glass and its application in serum sarcosine determination (2011), Indian J. Biotechnol., 10, 219-223.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme onto alkylamine and arylamine glass by crosslinking with glutaraldehyde for determination of serum sarcosine, method, overview. The enzyme shows a decrease in optimum pH and KM, and an increase in optimum temperature and time for linearity after immobilization Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.285
-
sarcosine immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
1.379
-
sarcosine immobilized enzyme on arylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
50
-
sarcosine free enzyme, pH 7.8, 37°C Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + H2O + O2 Arthrobacter sp.
-
glycine + formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.81
-
immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
2.02
-
immobilized enzyme on arylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
2.38
-
soluble enzyme, pH 7.8, 37°C Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + H2O + O2
-
Arthrobacter sp. glycine + formaldehyde + H2O2
-
?

Synonyms

Synonyms Comment Organism
sarcosine: O2 oxidoreductase
-
Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
free enzyme Arthrobacter sp.
37 40 immobilized enzyme Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
immobilized enzyme Arthrobacter sp.
9
-
free enzyme Arthrobacter sp.