Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme onto alkylamine and arylamine glass by crosslinking with glutaraldehyde for determination of serum sarcosine, method, overview. The enzyme shows a decrease in optimum pH and KM, and an increase in optimum temperature and time for linearity after immobilization | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.285 | - |
sarcosine | immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C | Arthrobacter sp. | |
1.379 | - |
sarcosine | immobilized enzyme on arylamine glass beads, pH 7.8, 37°C | Arthrobacter sp. | |
50 | - |
sarcosine | free enzyme, pH 7.8, 37°C | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | Arthrobacter sp. | - |
glycine + formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Arthrobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.81 | - |
immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C | Arthrobacter sp. |
2.02 | - |
immobilized enzyme on arylamine glass beads, pH 7.8, 37°C | Arthrobacter sp. |
2.38 | - |
soluble enzyme, pH 7.8, 37°C | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Arthrobacter sp. | glycine + formaldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sarcosine: O2 oxidoreductase | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
free enzyme | Arthrobacter sp. |
37 | 40 | immobilized enzyme | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
immobilized enzyme | Arthrobacter sp. |
9 | - |
free enzyme | Arthrobacter sp. |