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Literature summary for 1.5.3.1 extracted from

  • Tong, Y.; Xin, Y.; Yang, H.; Zhang, L.; Wang, W.
    Efficient improvement on stability of sarcosine oxidase via poly-lysine modification on enzyme surface (2014), Int. J. Biol. Macromol., 67, 140-146 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 Bacillus sp. BSD-8

General Stability

General Stability Organism
modified technique is successfully established for efficiently improving the stability of sarcosine oxidase via poly-Lys covalent binding onto enzymatic surface. Compared to the free enzyme, higher pH stability and thermostability of modified sarcosine oxidase is achieved with a series of characterization analysis. It is owing to that the loosening of three-dimensional configuration can be restricted by the enzymatic surface protection with poly-Lys and subsequently maintained a more stable enzyme structure. The changed microenvironment around the enzyme in themodification process can also contribute to the better stability Bacillus sp. BSD-8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
47.94
-
sarcosine 37°C, pH 8.0, poly-lysine modified enzyme Bacillus sp. BSD-8
141.65
-
sarcosine 37°C, pH 8.0, soluble enzyme Bacillus sp. BSD-8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43800
-
soluble enzyme, mass spectrum analysis Bacillus sp. BSD-8
48000
-
and 51700, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis Bacillus sp. BSD-8
51700
-
and 48000, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis Bacillus sp. BSD-8

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + H2O + O2 Bacillus sp. BSD-8
-
glycine + formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. BSD-8 Q6ITC6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. BSD-8

Storage Stability

Storage Stability Organism
37°C, pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% Bacillus sp. BSD-8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + H2O + O2
-
Bacillus sp. BSD-8 glycine + formaldehyde + H2O2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% Bacillus sp. BSD-8
60
-
10 min, residual activity of soluble enzyme is 2.3%, residual activity poly-lysine modified enzymeis 74.7% Bacillus sp. BSD-8

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 37°C, 2.5 h, more than 90% of initial activity of poly-lysine modified enzyme is maintained Bacillus sp. BSD-8