Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Bacillus sp. BSD-8 |
General Stability | Organism |
---|---|
modified technique is successfully established for efficiently improving the stability of sarcosine oxidase via poly-Lys covalent binding onto enzymatic surface. Compared to the free enzyme, higher pH stability and thermostability of modified sarcosine oxidase is achieved with a series of characterization analysis. It is owing to that the loosening of three-dimensional configuration can be restricted by the enzymatic surface protection with poly-Lys and subsequently maintained a more stable enzyme structure. The changed microenvironment around the enzyme in themodification process can also contribute to the better stability | Bacillus sp. BSD-8 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47.94 | - |
sarcosine | 37°C, pH 8.0, poly-lysine modified enzyme | Bacillus sp. BSD-8 | |
141.65 | - |
sarcosine | 37°C, pH 8.0, soluble enzyme | Bacillus sp. BSD-8 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43800 | - |
soluble enzyme, mass spectrum analysis | Bacillus sp. BSD-8 |
48000 | - |
and 51700, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis | Bacillus sp. BSD-8 |
51700 | - |
and 48000, modified enzyme covalently bound with about two or three poly-Lys, mass spectrum analysis | Bacillus sp. BSD-8 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | Bacillus sp. BSD-8 | - |
glycine + formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. BSD-8 | Q6ITC6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. BSD-8 |
Storage Stability | Organism |
---|---|
37°C, pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% | Bacillus sp. BSD-8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 8.0, 7 days, residual activity of soluble enzyme is 7.9%, residual activity poly-lysine modified enzyme is 95.6% | Bacillus sp. BSD-8 |
60 | - |
10 min, residual activity of soluble enzyme is 2.3%, residual activity poly-lysine modified enzymeis 74.7% | Bacillus sp. BSD-8 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 37°C, 2.5 h, more than 90% of initial activity of poly-lysine modified enzyme is maintained | Bacillus sp. BSD-8 |