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Literature summary for 1.5.3.1 extracted from

  • Ida, K.; Moriguchi, T.; Suzuki, H.
    Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96 (2005), Biochem. Biophys. Res. Commun., 333, 359-366 .
    View publication on PubMed
Search for more literature for 1.5.3.1

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with dimethylglycine and folinic acid, vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 1.9 M ammonium sulfate, and 10 mM CuSO4 Corynebacterium sp. U-96

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the delta subunit contains a single atom of zinc Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
 glycine + formaldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp. U-96
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration Corynebacterium sp. U-96

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 glycine + formaldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
heterotetramer x-ray crystallography Corynebacterium sp. U-96

Synonyms

Synonyms Comment Organism
heterotetrameric sarcosine oxidase
-
 Corynebacterium sp. U-96
TSOX
-
 Corynebacterium sp. U-96

Cofactor

Cofactor Comment Organism Structure
FAD the beta subunit contains FAD Corynebacterium sp. U-96
FMN the beta subunit contains FMN Corynebacterium sp. U-96
NAD+ the alpha subunit contains NAD+ Corynebacterium sp. U-96