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Literature summary for 1.5.3.13 extracted from

  • Takao, K.; Sugita, Y.; Shirahata, A.
    Assay of N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by ion-pair reversed phase high performance liquid chromatography (2010), Biol. Pharm. Bull., 33, 1089-1094.
    View publication on PubMed
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Application

Application Comment Organism
analysis assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Among the dansylpolyamines, N1,N11-didansylnorspermine is a useful substrate for the development of the N1-acetylpolyamine oxidase assay method. Contaminating polyamines in biological samples do not interfere with the method Rattus norvegicus
analysis assay method to measure N1-acetylpolyamine oxidase activity with N1,N11-didansylnorspermine as the substrate by high performance liquid chromatography. Applied to cell extract from Chinese hamster ovary cell samples, the PAO activity can easily be detected with as little as 10 microg of protein, which corresponds to 1105 cells. The method is rapid, sensitive and useful. Contaminating polyamines in biological samples do not interfere with the method Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
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Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1,N11-didansylnorspermine + O2 + H2O
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 Cricetulus griseus 1-dansylnorspermidine + 1-dansylamido-3-propanal + H2O2
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 ?
N1,N11-didansylnorspermine + O2 + H2O
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 Rattus norvegicus 1-dansylnorspermidine + 1-dansylamido-3-propanal + H2O2
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 ?