Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 DE3 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N1-bis(2,3-butadienyl)-1,4-butanediamine | i.e. MDL72527, competitive. If incubated for longer times, MDL72527 yields irreversible inhibition of the enzyme with a half-life of 15 min at 0.1 mM MDL72527 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
spermine | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99K82 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with spermidine and putrescine | Mus musculus | ? | - |
? | |
spermine + O2 + H2O | the mMSO catalytic mechanism is consistent with a simple four-step kinetic scheme. The enzyme is unable to oxidize other free or acetylated polyamines | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
mSMO | - |
Mus musculus |
spermine oxidase | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
spermine | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | non-covalently bound to the enzyme | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
N,N1-bis(2,3-butadienyl)-1,4-butanediamine | - |
Mus musculus |