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Literature summary for 1.5.3.21 extracted from
- Final steps in the catabolism of nicotine (2006), FEBS J., 273, 1528-1536.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
4-methylaminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans |  |
| 6.66 | - |
4-aminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46100, calculated, x * 46000, SDS-PAGE | Paenarthrobacter nicotinovorans |
| 46100 | - |
x * 46100, calculated, x * 46000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobutanoate + O2 + H2O | - |
Paenarthrobacter nicotinovorans | succinate semialdehyde + NH3 + H2O2 | - |
? | |
| 4-methylaminobutanoate + O2 + H2O | - |
Paenarthrobacter nicotinovorans | succinate semialdehyde + methylamine + H2O2 | - |
? | |
| additional information | no substrates: spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyric acid, methylaminopropionnitrile, methylaminopropylamine | Paenarthrobacter nicotinovorans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46100, calculated, x * 46000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MAO | - |
Paenarthrobacter nicotinovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Paenarthrobacter nicotinovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 878 | - |
4-aminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans | |
| 1230 | - |
4-methylaminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
- |
Paenarthrobacter nicotinovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | noncovalently bound | Paenarthrobacter nicotinovorans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate | Paenarthrobacter nicotinovorans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 131 | - |
4-aminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans | |
| 490 | - |
4-methylaminobutanoate | pH 9.8, 22°C | Paenarthrobacter nicotinovorans | |
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Release 2023.1 (January 2023)
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