BRENDA - Enzyme Database show
show all sequences of 1.5.3.21

Final steps in the catabolism of nicotine

Chiribau, C.; Mihasan, M.; Ganas, P.; Igloi, G.; Artenie, V.; Brandsch, R.; FEBS J. 273, 1528-1536 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
6.66
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
46100
-
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paenarthrobacter nicotinovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + O2 + H2O
-
719443
Paenarthrobacter nicotinovorans
succinate semialdehyde + NH3 + H2O2
-
-
-
?
4-methylaminobutanoate + O2 + H2O
-
719443
Paenarthrobacter nicotinovorans
succinate semialdehyde + methylamine + H2O2
-
-
-
?
additional information
no substrates: spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyric acid, methylaminopropionnitrile, methylaminopropylamine
719443
Paenarthrobacter nicotinovorans
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Paenarthrobacter nicotinovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
878
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
1230
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
-
-
Paenarthrobacter nicotinovorans
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
noncovalently bound
Paenarthrobacter nicotinovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
noncovalently bound
Paenarthrobacter nicotinovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
6.66
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
46100
-
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + O2 + H2O
-
719443
Paenarthrobacter nicotinovorans
succinate semialdehyde + NH3 + H2O2
-
-
-
?
4-methylaminobutanoate + O2 + H2O
-
719443
Paenarthrobacter nicotinovorans
succinate semialdehyde + methylamine + H2O2
-
-
-
?
additional information
no substrates: spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyric acid, methylaminopropionnitrile, methylaminopropylamine
719443
Paenarthrobacter nicotinovorans
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 46100, calculated, x * 46000, SDS-PAGE
Paenarthrobacter nicotinovorans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Paenarthrobacter nicotinovorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
878
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
1230
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.8
-
-
Paenarthrobacter nicotinovorans
General Information
General Information
Commentary
Organism
physiological function
in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate
Paenarthrobacter nicotinovorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate
Paenarthrobacter nicotinovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
131
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
490
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
131
-
4-aminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
490
-
4-methylaminobutanoate
pH 9.8, 22°C
Paenarthrobacter nicotinovorans
Other publictions for EC 1.5.3.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719443
Chiribau
Final steps in the catabolism ...
Paenarthrobacter nicotinovorans
FEBS J.
273
1528-1536
2006
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
-
-
-
3
1
1
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
3
1
1
-
-
2
1
-
-
-
-
1
1
-
2
2