Literature summary for 1.5.3.24 extracted from

Kvalnes-Krick, K.; Jorns, M.S.
Bacterial sarcosine oxidase comparison of two multisubunit enzymes containing both covalent and noncovalent flavin (1986), Biochemistry, 25, 6061-6069 .

Search for more literature for 1.5.3.24

General Stability

General Stability	Organism
no activity loss is detected after 3 cycles of freezing and thawing within 1 week	Corynebacterium sp. P-1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
168000	-	gel filtration	Corynebacterium sp. P-1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2	Corynebacterium sp. P-1	-	glycine + 5,10-methylenetetrahydrofolate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium sp. P-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Ultrogel AcA gel filtration, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and DEAE Sephacel column chromatography	Corynebacterium sp. P-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.33	-	cell lysate, at pH 8.3 and 37°C	Corynebacterium sp. P-1
12.2	-	after 37.1fold purification, at pH 8.3 and 37°C	Corynebacterium sp. P-1

Storage Stability

Storage Stability	Organism
-70°C, purified enzyme in 10 mM potassium phosphate, pH 8.0, at least 1 year, no loss of activity	Corynebacterium sp. P-1
0°C, purified enzyme in 10 mM potassium phosphate, pH 8.0, 2 weeks, no loss of activity	Corynebacterium sp. P-1
25°C, purified enzyme in 10 mM potassium phosphate, pH 8.0, 24 h, no loss of activity	Corynebacterium sp. P-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2	-	Corynebacterium sp. P-1	glycine + 5,10-methylenetetrahydrofolate + H2O2	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	1 * 100000 + 1 * 42000 + 1 * 20000 + 1 * 6000, SDS-PAGE	Corynebacterium sp. P-1

Synonyms

Synonyms	Comment	Organism
sarcosine oxidase P	-	Corynebacterium sp. P-1

Cofactor

Cofactor	Comment	Organism	Structure
FAD	contains 1 mol of noncovalently bound Flavin (FAD) plus 1 mol of covalently bound flavin [8alpha-(N3-histidyl)-FAD] per mole of enzyme	Corynebacterium sp. P-1

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Release 2023.1 (January 2023)