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Literature summary for 1.5.3.24 extracted from
- Preparation and properties of recombinant corynebacterial sarcosine oxidase evidence for posttranslational modification during turnover with sarcosine (1993), Biochemistry, 32, 11132-11142 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1-Blue/pLJC305 cells | Corynebacterium sp. P-1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. P-1 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium sp. P-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Ultrogel AC A 34 Sepharose column chromatography, phenyl-Sepharose column chromatography, and DEAE-Sephacel column chromatography | Corynebacterium sp. P-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. P-1 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 1 * 100000 + 1 * 42000 + 1 * 20000 + 1 * 6000, SDS-PAGE | Corynebacterium sp. P-1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Corynebacterium sp. P-1 | |
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