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Literature summary for 1.5.3.24 extracted from
- Identification of the covalent flavin attachment site in sarcosine oxidase (1998), Biochemistry, 37, 2089-2095 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL-1 Blue cells | Corynebacterium sp. P-1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H175A | the mutant contains 1 mol of covalently bound flavin (FMN) and exhibits catalytic activity similar to the wild type enzyme | Corynebacterium sp. P-1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. P-1 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium sp. P-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium sp. P-1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
mutant enzyme H175A, pH and temperature not specified in the publication | Corynebacterium sp. P-1 |
| 8.6 | - |
wild type enzyme, pH and temperature not specified in the publication | Corynebacterium sp. P-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. P-1 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | - |
Corynebacterium sp. P-1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sarcosine oxidase | - |
Corynebacterium sp. P-1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the wild type enzyme contains 1 mol of noncovalently bound FAD | Corynebacterium sp. P-1 | |
| FMN | the wild type enzyme contains 1 mol of covalently bound FMN attached to subunit beta | Corynebacterium sp. P-1 | |
| NAD+ | the wild type enzyme contains 1 mol of noncovalently bound NAD+ | Corynebacterium sp. P-1 | |
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