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Literature summary for 1.5.3.24 extracted from

  • Suzuki, M.
    Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96 (1981), J. Biochem., 89, 599-607 .
    View publication on PubMed
Search for more literature for 1.5.3.24

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 0.01 mM Corynebacterium sp. U-96
acetaldehyde about 50% inhibition at 20 mM Corynebacterium sp. U-96
acetate about 50% inhibition at 20 mM Corynebacterium sp. U-96
Ag+ complete inhibition at 2 mM Corynebacterium sp. U-96
Cd2+ 7% residual activity at 2 mM Corynebacterium sp. U-96
Co2+ 53% residual activity at 2 mM Corynebacterium sp. U-96
Cu2+ complete inhibition at 2 mM Corynebacterium sp. U-96
Fe2+ 66% residual activity at 2 mM Corynebacterium sp. U-96
formaldehyde about 50% inhibition at 20 mM Corynebacterium sp. U-96
Hg2+ complete inhibition at 2 mM Corynebacterium sp. U-96
iodoacetamide 4% residual activity at 2 mM Corynebacterium sp. U-96
KCN 45% residual activity at 2 mM Corynebacterium sp. U-96
Mn2+ 63% residual activity at 2 mM Corynebacterium sp. U-96
additional information there is no inhibitory effect with metal ions and chemicals such as Ca2+, Mg2+, Fe3+, alpha,alpha'-dipyridyl, EDTA, 2-mercaptoethanol, glutathione (reduced form), dithiothreitol, NaN3, NaF, and NaAsO2 Corynebacterium sp. U-96
N-ethylmaleimide 20% residual activity at 2 mM Corynebacterium sp. U-96
Ni2+ 76% residual activity at 2 mM Corynebacterium sp. U-96
o-phenanthroline 53% residual activity at 2 mM Corynebacterium sp. U-96
propionate about 50% inhibition at 20 mM Corynebacterium sp. U-96
sodium lauryl sulfate complete inhibition at 2 mM Corynebacterium sp. U-96
Zn2+ 7% residual activity at 2 mM Corynebacterium sp. U-96

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 O2 at pH 7.7 and 37°C Corynebacterium sp. U-96
0.31
-
 2,6-dichlorophenolindophenol at pH 7.7 and 37°C Corynebacterium sp. U-96
1.67
-
 phenazine methosulfate at pH 7.7 and 37°C Corynebacterium sp. U-96
2.5
-
 ferricyanide at pH 7.7 and 37°C Corynebacterium sp. U-96
3.4
-
 sarcosine at pH 8.3 and 37°C Corynebacterium sp. U-96
3.8
-
 sarcosine at pH 7.0 and 37°C Corynebacterium sp. U-96
8.7
-
 N-Methyl-L-alanine at pH 8.3 and 37°C Corynebacterium sp. U-96
11.4
-
 N-ethyl-glycine at pH 7.0 and 37°C Corynebacterium sp. U-96

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
174000
-
 gel filtration Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp. U-96
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose colum chromatography, QAE-Sephadex A-50 gel filtration, DEAE Sephadex A-50 gel filtration, Ultrogel AcA34 gel filtration, and creatine-AH-Sepharose 4B colum chromatography Corynebacterium sp. U-96

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
 crude enzyme, at pH 8.3 and 37°C Corynebacterium sp. U-96
8.93
-
 after 23.5fold purification, at pH 8.3 and 37°C Corynebacterium sp. U-96

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information oxygen cannot be replaced by methylene blue or cytochrome c. The enzyme shows no activity with N,N-dimethylglycine, glycine, betaine, choline, 1,3-dimethylurea, 1-methylguanidine, 1-methylhydantoin, sarcosyl-glycine, and sarcosyl-sarcosine Corynebacterium sp. U-96 ?
-
-
N-ethyl-glycine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 ?
-
 ?
N-methyl-L-alanine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 ?
-
 ?
sarcosine + 5,6,7,8-tetrahydrofolate + 2,6-dichlorophenolindophenol
-
 Corynebacterium sp. U-96 ?
-
 ?
sarcosine + 5,6,7,8-tetrahydrofolate + ferricyanide
-
 Corynebacterium sp. U-96 ?
-
 ?
sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?
sarcosine + 5,6,7,8-tetrahydrofolate + phenazine methosulfate
-
 Corynebacterium sp. U-96 ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 1 * 110000 + 1 * 44000 + 1 * 21000 + 1 * 10000, SDS-PAGE Corynebacterium sp. U-96

Synonyms

Synonyms Comment Organism
sarcosine: oxygen oxidoreductase (demethylating)
-
 Corynebacterium sp. U-96

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 in glycylglycine buffer Corynebacterium sp. U-96

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the enzyme activity is completely lost on heating at 45°C for 10 min Corynebacterium sp. U-96

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 N-Methyl-L-alanine at pH 7.0 and 37°C Corynebacterium sp. U-96
2.1
-
 N-Methyl-L-alanine at pH 8.3 and 37°C Corynebacterium sp. U-96
2.2
-
 N-ethyl-glycine at pH 7.0 and 37°C Corynebacterium sp. U-96
5.8
-
 sarcosine at pH 8.3 and 37°C Corynebacterium sp. U-96

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 in potassium phosphate buffer Corynebacterium sp. U-96
8 8.5 in glycylglycine buffer Corynebacterium sp. U-96

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 the enzyme remains stable for 24 h at pH 7.0-10.0 and 5°C Corynebacterium sp. U-96

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains both 1 mol of non-covalently bound FAD and 1 mol of covalently bound FAD per mol of enzyme Corynebacterium sp. U-96