BRENDA - Enzyme Database show
show all sequences of 1.5.3.4

epsilon-Alkyllysinase. New assay method, purification, and biological significance

Paik, W.K.; Kim, S.; Arch. Biochem. Biophys. 165, 369-378 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
10% glycerol protects enzyme from inactivation during Sephadex G-200 column chromatography
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.05
-
N6-methyl-L-lysine
crude extract
Rattus norvegicus
1.9
-
N6-methyl-L-lysine
partially purified enzyme
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
exclusive localization
Rattus norvegicus
5739
-
particle-bound
-
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
200000
gel filtration
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
16fold partial purification; enzyme from kidney
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
N6-methyl-L-lysine + H2O + O2 = L-lysine + formaldehyde + H2O2
mechanism
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
low enzyme activity in young rat, increases to the adult level, highest level of demethylation activity
Rattus norvegicus
-
liver
low enzyme activity in young rat, increases to the adult level, after kidney next highest level of demethylation activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
values in dependence of different incubation mixtures
Rattus norvegicus
0.0283
-
11fold purified enzyme
Rattus norvegicus
0.0433
-
16fold purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
free alpha-amino group is not required for susceptibility
392427
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
additional information
not: delta-N-monomethyl-L-ornithine
392427
Rattus norvegicus
?
-
-
-
-
additional information
demethylation involves an electron transport system: FAD is the physiological electron acceptor, phenazine methosulfate as artificial and more efficient electron acceptor than FAD, final electron acceptor is oxygen or 2,6-dichlorophenolindophenol
392427
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
-
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-D-lysine + H2O + O2
11% of the activity with epsilon-N-methyl-L-lysine
392427
Rattus norvegicus
D-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
reaction in reverse direction, i.e. methylation of L-lysine, is probably catalyzed by protein methylase III
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
equimolar amounts of L-lysine and formaldehyde
392427
Rattus norvegicus
ir
N6-methyl-L-lysine + O2 + H2O
enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
equimolar amounts of L-lysine and formaldehyde
392427
Rattus norvegicus
ir
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
4 min: 70% loss of activity, 10 min: complete loss of activity
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6: about 35% of activity maximum, pH 8: about 50% of activity maximum
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
physiological electron acceptor, not required with whole homogenate, but required for partially purified enzyme
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
physiological electron acceptor, not required with whole homogenate, but required for partially purified enzyme
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
10% glycerol protects enzyme from inactivation during Sephadex G-200 column chromatography
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.05
-
N6-methyl-L-lysine
crude extract
Rattus norvegicus
1.9
-
N6-methyl-L-lysine
partially purified enzyme
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
exclusive localization
Rattus norvegicus
5739
-
particle-bound
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
200000
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
16fold partial purification; enzyme from kidney
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
low enzyme activity in young rat, increases to the adult level, highest level of demethylation activity
Rattus norvegicus
-
liver
low enzyme activity in young rat, increases to the adult level, after kidney next highest level of demethylation activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
values in dependence of different incubation mixtures
Rattus norvegicus
0.0283
-
11fold purified enzyme
Rattus norvegicus
0.0433
-
16fold purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
free alpha-amino group is not required for susceptibility
392427
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
additional information
not: delta-N-monomethyl-L-ornithine
392427
Rattus norvegicus
?
-
-
-
-
additional information
demethylation involves an electron transport system: FAD is the physiological electron acceptor, phenazine methosulfate as artificial and more efficient electron acceptor than FAD, final electron acceptor is oxygen or 2,6-dichlorophenolindophenol
392427
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
-
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-D-lysine + H2O + O2
11% of the activity with epsilon-N-methyl-L-lysine
392427
Rattus norvegicus
D-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
reaction in reverse direction, i.e. methylation of L-lysine, is probably catalyzed by protein methylase III
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
equimolar amounts of L-lysine and formaldehyde
392427
Rattus norvegicus
ir
N6-methyl-L-lysine + O2 + H2O
enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen
392427
Rattus norvegicus
L-lysine + formaldehyde + H2O2
equimolar amounts of L-lysine and formaldehyde
392427
Rattus norvegicus
ir
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
4 min: 70% loss of activity, 10 min: complete loss of activity
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6: about 35% of activity maximum, pH 8: about 50% of activity maximum
Rattus norvegicus
Other publictions for EC 1.5.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485480
Paik
Enzymatic methylation and deme ...
Rattus norvegicus
Methods Enzymol.
106
274-287
1984
-
-
-
1
-
-
5
1
-
-
-
2
-
1
-
-
1
-
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
1
-
-
-
2
-
-
-
1
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392427
Paik
epsilon-Alkyllysinase. New ass ...
Rattus norvegicus
Arch. Biochem. Biophys.
165
369-378
1974
-
-
-
-
-
1
1
2
2
-
1
-
-
1
-
-
1
1
-
2
3
-
7
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
2
2
-
1
-
-
-
-
1
-
2
3
-
7
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
392428
Kim
Epsilon-alkyllysinase. Purific ...
Canis lupus familiaris, Cavia porcellus, Columba sp., Felis catus, Gallus gallus, Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
239
3790-3796
1964
1
-
-
-
-
-
11
3
2
-
-
-
-
9
-
-
1
-
-
18
1
-
19
-
1
-
1
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
11
-
3
2
-
-
-
-
-
-
1
-
18
1
-
19
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-