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show all sequences of 1.5.3.4

Epsilon-alkyllysinase. Purification and properties of the enzyme

Kim, S.; Benoiton, L.; Woon Ki Paik; J. Biol. Chem. 239, 3790-3796 (1964)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphate
stimulates enzyme in prepared mitochondrial fraction, but inhibits activity in crude homogenate
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ascorbic acid
moderate inhibition
Rattus norvegicus
Co2+
strong inhibitory effect
Rattus norvegicus
FAD
moderate inhibition
Rattus norvegicus
FMN
moderate inhibition
Rattus norvegicus
KCN
1.3 mM, about 40% inhibition
Rattus norvegicus
Mn2+
moderate inhibition
Rattus norvegicus
additional information
not inhibited by semicarbazide
Rattus norvegicus
NADH
moderate inhibition
Rattus norvegicus
Ni2+
strong inhibitory effect
Rattus norvegicus
phosphate
inhibits activity in crude homogenate, but stimulates enzyme in prepared mitochondrial fraction
Rattus norvegicus
Zn2+
strong inhibitory effect
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.87
-
N6,N6-dimethyl-L-lysine
-
Rattus norvegicus
1.63
-
N6-methyl-L-lysine
-
Rattus norvegicus
5.95
-
N6-ethyl-L-lysine
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
60% of the activity in rat kidney is present in the mitochondrial fraction
Rattus norvegicus
5739
-
particle-bound
-
Rattus norvegicus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Canis lupus familiaris
-
-
-
Cavia porcellus
-
-
-
Columba sp.
-
-
-
Felis catus
-
low activity
-
Gallus gallus
-
-
-
Mesocricetus auratus
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
2.78fold partial purification; enzyme from kidney
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Canis lupus familiaris
-
kidney
-
Cavia porcellus
-
kidney
higher activity than in liver
Columba sp.
-
kidney
higher activity than in liver
Felis catus
-
kidney
higher activity than in liver
Gallus gallus
-
kidney
higher activity than in liver
Mesocricetus auratus
-
kidney
-
Mus musculus
-
kidney
higher activity than in liver
Oryctolagus cuniculus
-
kidney
higher activity than in liver; highest activity
Rattus norvegicus
-
liver
-
Canis lupus familiaris
-
liver
low activity
Cavia porcellus
-
liver
low activity; lower activity than in kidney
Columba sp.
-
liver
lower activity than in kidney
Felis catus
-
liver
low activity; lower activity than in kidney
Gallus gallus
-
liver
lower activity than in kidney
Mesocricetus auratus
-
liver
-
Mus musculus
-
liver
lower activity than in kidney
Oryctolagus cuniculus
-
liver
lower activity than in kidney
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0044
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
15% of the activity with N6-methyl-L-lysine
392428
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
6-(methylamino)-2-oxohexanoic acid + H2O + O2
free alpha-amino group is not required for susceptibility
392428
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
additional information
not: delta-N-monomethyl-L-ornithine
392428
Rattus norvegicus
?
-
-
-
-
additional information
not: several acyl derivatives, alpha-N-methyl-L-lysine
392428
Rattus norvegicus
?
-
-
-
-
additional information
activity restricted to epsilon-N-alkyllysine derivatives
392428
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
46% of the activity with N6-methyl-L-lysine, it may be oxidized by a different enzyme
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6,N6-dimethyl-L-lysine + O2 + H2O
enzyme consumes per molecule N6,N6-dimethyl-L-lysine one molecule of oxygen
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-ethyl-L-lysine + H2O + O2
about 50% of the activity with N6-methyl-L-lysine
392428
Rattus norvegicus
L-lysine + acetaldehyde + H2O2
-
-
-
?
N6-methyl-D-lysine + H2O + O2
11% of the activity with epsilon-N-methyl-L-lysine
392428
Rattus norvegicus
D-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Gallus gallus
L-lysine + formaldehyde + H2O2
-
392428
Gallus gallus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Cavia porcellus
L-lysine + formaldehyde + H2O2
-
392428
Cavia porcellus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Mus musculus
L-lysine + formaldehyde + H2O2
-
392428
Mus musculus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Oryctolagus cuniculus
L-lysine + formaldehyde + H2O2
-
392428
Oryctolagus cuniculus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Canis lupus familiaris
L-lysine + formaldehyde + H2O2
-
392428
Canis lupus familiaris
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Mesocricetus auratus
L-lysine + formaldehyde + H2O2
-
392428
Mesocricetus auratus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Felis catus
L-lysine + formaldehyde + H2O2
-
392428
Felis catus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Columba sp.
L-lysine + formaldehyde + H2O2
-
392428
Columba sp.
?
N6-methyl-L-lysine + O2 + H2O
highest enzyme activity
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
392428
Rattus norvegicus
?
N6-methyl-L-lysine + O2 + H2O
enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
392428
Rattus norvegicus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Rattus norvegicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
enzyme is resistant to heat, far more stable in presence of substrates
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
partially purified enzyme
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.8
pH 6: about 10% of activity maximum, pH 7.8: about 15% of activity maximum
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme is most likely a flavoprotein
Rattus norvegicus
additional information
no cofactor requirement
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphate
stimulates enzyme in prepared mitochondrial fraction, but inhibits activity in crude homogenate
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme is most likely a flavoprotein
Rattus norvegicus
additional information
no cofactor requirement
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ascorbic acid
moderate inhibition
Rattus norvegicus
Co2+
strong inhibitory effect
Rattus norvegicus
FAD
moderate inhibition
Rattus norvegicus
FMN
moderate inhibition
Rattus norvegicus
KCN
1.3 mM, about 40% inhibition
Rattus norvegicus
Mn2+
moderate inhibition
Rattus norvegicus
additional information
not inhibited by semicarbazide
Rattus norvegicus
NADH
moderate inhibition
Rattus norvegicus
Ni2+
strong inhibitory effect
Rattus norvegicus
phosphate
inhibits activity in crude homogenate, but stimulates enzyme in prepared mitochondrial fraction
Rattus norvegicus
Zn2+
strong inhibitory effect
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.87
-
N6,N6-dimethyl-L-lysine
-
Rattus norvegicus
1.63
-
N6-methyl-L-lysine
-
Rattus norvegicus
5.95
-
N6-ethyl-L-lysine
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
60% of the activity in rat kidney is present in the mitochondrial fraction
Rattus norvegicus
5739
-
particle-bound
-
Rattus norvegicus
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
2.78fold partial purification; enzyme from kidney
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Canis lupus familiaris
-
kidney
-
Cavia porcellus
-
kidney
higher activity than in liver
Columba sp.
-
kidney
higher activity than in liver
Felis catus
-
kidney
higher activity than in liver
Gallus gallus
-
kidney
higher activity than in liver
Mesocricetus auratus
-
kidney
-
Mus musculus
-
kidney
higher activity than in liver
Oryctolagus cuniculus
-
kidney
higher activity than in liver; highest activity
Rattus norvegicus
-
liver
-
Canis lupus familiaris
-
liver
low activity
Cavia porcellus
-
liver
low activity; lower activity than in kidney
Columba sp.
-
liver
lower activity than in kidney
Felis catus
-
liver
low activity; lower activity than in kidney
Gallus gallus
-
liver
lower activity than in kidney
Mesocricetus auratus
-
liver
-
Mus musculus
-
liver
lower activity than in kidney
Oryctolagus cuniculus
-
liver
lower activity than in kidney
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0044
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
15% of the activity with N6-methyl-L-lysine
392428
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
6-(methylamino)-2-oxohexanoic acid + H2O + O2
free alpha-amino group is not required for susceptibility
392428
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
additional information
not: delta-N-monomethyl-L-ornithine
392428
Rattus norvegicus
?
-
-
-
-
additional information
not: several acyl derivatives, alpha-N-methyl-L-lysine
392428
Rattus norvegicus
?
-
-
-
-
additional information
activity restricted to epsilon-N-alkyllysine derivatives
392428
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
46% of the activity with N6-methyl-L-lysine, it may be oxidized by a different enzyme
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6,N6-dimethyl-L-lysine + O2 + H2O
enzyme consumes per molecule N6,N6-dimethyl-L-lysine one molecule of oxygen
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-ethyl-L-lysine + H2O + O2
about 50% of the activity with N6-methyl-L-lysine
392428
Rattus norvegicus
L-lysine + acetaldehyde + H2O2
-
-
-
?
N6-methyl-D-lysine + H2O + O2
11% of the activity with epsilon-N-methyl-L-lysine
392428
Rattus norvegicus
D-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Gallus gallus
L-lysine + formaldehyde + H2O2
-
392428
Gallus gallus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Cavia porcellus
L-lysine + formaldehyde + H2O2
-
392428
Cavia porcellus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Mus musculus
L-lysine + formaldehyde + H2O2
-
392428
Mus musculus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Oryctolagus cuniculus
L-lysine + formaldehyde + H2O2
-
392428
Oryctolagus cuniculus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Canis lupus familiaris
L-lysine + formaldehyde + H2O2
-
392428
Canis lupus familiaris
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Mesocricetus auratus
L-lysine + formaldehyde + H2O2
-
392428
Mesocricetus auratus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Felis catus
L-lysine + formaldehyde + H2O2
-
392428
Felis catus
?
N6-methyl-L-lysine + O2 + H2O
-
392428
Columba sp.
L-lysine + formaldehyde + H2O2
-
392428
Columba sp.
?
N6-methyl-L-lysine + O2 + H2O
highest enzyme activity
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
392428
Rattus norvegicus
?
N6-methyl-L-lysine + O2 + H2O
enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen
392428
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
392428
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Rattus norvegicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
enzyme is resistant to heat, far more stable in presence of substrates
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
partially purified enzyme
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.8
pH 6: about 10% of activity maximum, pH 7.8: about 15% of activity maximum
Rattus norvegicus
Other publictions for EC 1.5.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485480
Paik
Enzymatic methylation and deme ...
Rattus norvegicus
Methods Enzymol.
106
274-287
1984
-
-
-
1
-
-
5
1
-
-
-
2
-
1
-
-
1
-
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
1
-
-
-
2
-
-
-
1
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392427
Paik
epsilon-Alkyllysinase. New ass ...
Rattus norvegicus
Arch. Biochem. Biophys.
165
369-378
1974
-
-
-
-
-
1
1
2
2
-
1
-
-
1
-
-
1
1
-
2
3
-
7
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
2
2
-
1
-
-
-
-
1
-
2
3
-
7
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
392428
Kim
Epsilon-alkyllysinase. Purific ...
Canis lupus familiaris, Cavia porcellus, Columba sp., Felis catus, Gallus gallus, Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
239
3790-3796
1964
1
-
-
-
-
-
11
3
2
-
-
-
-
9
-
-
1
-
-
18
1
-
19
-
1
-
1
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
11
-
3
2
-
-
-
-
-
-
1
-
18
1
-
19
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-