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show all sequences of 1.5.3.4

Enzymatic methylation and demethylation of protein-bound lysine residues

Paik, W.K.; DiMaria, P.; Methods Enzymol. 106, 274-287 (1984)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
inhibitory toward the partially purified enzyme
Rattus norvegicus
Co2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
KCN
1.3 mM, about 40% inhibition
Rattus norvegicus
Ni2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
Zn2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.05
-
N6-methyl-L-lysine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
Rattus norvegicus
methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase
demethylated lysine residues + formaldehyde + H2O2
-
Rattus norvegicus
ir
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
Rattus norvegicus
protein: e.g. histones
demethylated lysine residues + formaldehyde + H2O2
-
Rattus norvegicus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
16fold partial purification; enzyme from kidney
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00106
-
N6-methyl-L-lysyl-histone
Rattus norvegicus
0.0283
-
N6-methyl-L-lysine
Rattus norvegicus
0.0433
-
16fold purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
-
485480
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase
485480
Rattus norvegicus
demethylated lysine residues + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
protein: e.g. histones
485480
Rattus norvegicus
demethylated lysine residues + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
additional information
not: delta-N-monomethyl-L-ornithine
485480
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
-
485480
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
-
485480
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
N6-methyl-L-lysyl-histone + O2 + H2O
-
485480
Rattus norvegicus
histone + formaldehyde + H2O2
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
phosphate buffer
Rattus norvegicus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
inhibitory toward the partially purified enzyme
Rattus norvegicus
Co2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
KCN
1.3 mM, about 40% inhibition
Rattus norvegicus
Ni2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
Zn2+
inhibitory at 1.2 mM concentration
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.05
-
N6-methyl-L-lysine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
Rattus norvegicus
methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase
demethylated lysine residues + formaldehyde + H2O2
-
Rattus norvegicus
ir
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
Rattus norvegicus
protein: e.g. histones
demethylated lysine residues + formaldehyde + H2O2
-
Rattus norvegicus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
16fold partial purification; enzyme from kidney
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00106
-
N6-methyl-L-lysyl-histone
Rattus norvegicus
0.0283
-
N6-methyl-L-lysine
Rattus norvegicus
0.0433
-
16fold purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(methylamino)-2-oxohexanoic acid + H2O + O2
-
485480
Rattus norvegicus
6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
-
-
?
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase
485480
Rattus norvegicus
demethylated lysine residues + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O
protein: e.g. histones
485480
Rattus norvegicus
demethylated lysine residues + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
additional information
not: delta-N-monomethyl-L-ornithine
485480
Rattus norvegicus
?
-
-
-
-
N6,N6-dimethyl-L-lysine + O2 + H2O
-
485480
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
-
-
?
N6-methyl-L-lysine + O2 + H2O
-
485480
Rattus norvegicus
L-lysine + formaldehyde + H2O2
-
485480
Rattus norvegicus
ir
N6-methyl-L-lysyl-histone + O2 + H2O
-
485480
Rattus norvegicus
histone + formaldehyde + H2O2
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
phosphate buffer
Rattus norvegicus
Other publictions for EC 1.5.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485480
Paik
Enzymatic methylation and deme ...
Rattus norvegicus
Methods Enzymol.
106
274-287
1984
-
-
-
1
-
-
5
1
-
-
-
2
-
1
-
-
1
-
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
1
-
-
-
2
-
-
-
1
-
1
3
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392427
Paik
epsilon-Alkyllysinase. New ass ...
Rattus norvegicus
Arch. Biochem. Biophys.
165
369-378
1974
-
-
-
-
-
1
1
2
2
-
1
-
-
1
-
-
1
1
-
2
3
-
7
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
2
2
-
1
-
-
-
-
1
-
2
3
-
7
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
392428
Kim
Epsilon-alkyllysinase. Purific ...
Canis lupus familiaris, Cavia porcellus, Columba sp., Felis catus, Gallus gallus, Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
239
3790-3796
1964
1
-
-
-
-
-
11
3
2
-
-
-
-
9
-
-
1
-
-
18
1
-
19
-
1
-
1
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
11
-
3
2
-
-
-
-
-
-
1
-
18
1
-
19
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-