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Literature summary for 1.5.3.5 extracted from

  • Yu, H.; Tang, H.; Zhu, X.; Li, Y.; Xu, P.
    Molecular mechanism of nicotine degradation by a newly isolated strain, Ochrobactrum sp. strain SJY1 (2015), Appl. Environ. Microbiol., 81, 272-281 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Ochrobactrum sp. SJY1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24.6
-
(S)-6-hydroxynicotine pH 8.0, 25┬░C Ochrobactrum sp. SJY1

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the reaction progresses without the participation of metal ions Ochrobactrum sp. SJY1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2 Ochrobactrum sp. SJY1 the enzyme is involved in degradation of nicotine 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp. SJY1 A0A075XFI8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ochrobactrum sp. SJY1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-6-hydroxynicotine + H2O + O2
-
Ochrobactrum sp. SJY1 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
?
(S)-6-hydroxynicotine + H2O + O2 the enzyme is involved in degradation of nicotine Ochrobactrum sp. SJY1 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
?

Synonyms

Synonyms Comment Organism
6-hydroxy-L-nicotine oxidase
-
Ochrobactrum sp. SJY1
6HLNO
-
Ochrobactrum sp. SJY1
VppB
-
Ochrobactrum sp. SJY1

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
-
Ochrobactrum sp. SJY1

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
15 65 15┬░C: about 40% of maximal activity, 65┬░C: about 55% of maximal activity Ochrobactrum sp. SJY1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.8
-
(S)-6-hydroxynicotine pH 8.0, 25┬░C Ochrobactrum sp. SJY1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Ochrobactrum sp. SJY1

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 65% of maximal activity, pH 9.0: about 25% of maximal activity Ochrobactrum sp. SJY1

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound to the enzyme Ochrobactrum sp. SJY1