BRENDA - Enzyme Database
show all sequences of 1.5.3.7

Lysine biosynthesis in Rhodotorula glutinis: properties of pipecolic acid oxidase

Kinzel, J.J.; Bhattacharjee, J.K.; J. Bacteriol. 151, 1073-1077 (1982)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
100% inhibition at 1 mM
Rhodotorula glutinis
hydroxylamine
15% inhibition at 1 mM
Rhodotorula glutinis
KCN
20% inhibition at 1 mM
Rhodotorula glutinis
L-proline
competitive inhibitor
Rhodotorula glutinis
p-chloromercuribenzoate
100% inhibition at 1 mM
Rhodotorula glutinis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7
-
L-pipecolic acid
-
Rhodotorula glutinis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 43000, SDS-PAGE
Rhodotorula glutinis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-pipecolate + O2
Rhodotorula glutinis
-
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
the product reacts with water to form 2-aminoadipate 6-semialdehyde
Rhodotorula glutinis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodotorula glutinis
-
strains wild-type, MU-1 and lys5
-
Storage Stability
Storage Stability
Organism
-70°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several weeks, 100% activity
Rhodotorula glutinis
4°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several days, 100% activity
Rhodotorula glutinis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-pipecolate + O2
-
392478
Rhodotorula glutinis
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
?
L-pipecolate + O2
-
392478
Rhodotorula glutinis
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
the product reacts with water to form 2-aminoadipate 6-semialdehyde
392478
Rhodotorula glutinis
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Rhodotorula glutinis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min 20% activity, bovine serum albumin at 1 mg per ml protects from thermal inactivation
Rhodotorula glutinis
53
-
complete inactivation
Rhodotorula glutinis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rhodotorula glutinis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
11
-
L-proline
-
Rhodotorula glutinis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
100% inhibition at 1 mM
Rhodotorula glutinis
hydroxylamine
15% inhibition at 1 mM
Rhodotorula glutinis
KCN
20% inhibition at 1 mM
Rhodotorula glutinis
L-proline
competitive inhibitor
Rhodotorula glutinis
p-chloromercuribenzoate
100% inhibition at 1 mM
Rhodotorula glutinis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
11
-
L-proline
-
Rhodotorula glutinis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7
-
L-pipecolic acid
-
Rhodotorula glutinis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
1 * 43000, SDS-PAGE
Rhodotorula glutinis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-pipecolate + O2
Rhodotorula glutinis
-
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
the product reacts with water to form 2-aminoadipate 6-semialdehyde
Rhodotorula glutinis
?
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several weeks, 100% activity
Rhodotorula glutinis
4°C, Tris-HCl buffer pH 8.7, 1 mM 2-mercaptoethanol, several days, 100% activity
Rhodotorula glutinis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-pipecolate + O2
-
392478
Rhodotorula glutinis
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
-
-
-
?
L-pipecolate + O2
-
392478
Rhodotorula glutinis
2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2
the product reacts with water to form 2-aminoadipate 6-semialdehyde
392478
Rhodotorula glutinis
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Rhodotorula glutinis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min 20% activity, bovine serum albumin at 1 mg per ml protects from thermal inactivation
Rhodotorula glutinis
53
-
complete inactivation
Rhodotorula glutinis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rhodotorula glutinis
Other publictions for EC 1.5.3.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742960
Natarajan
Evidence for pipecolate oxida ...
Homo sapiens
J. Cell. Biochem.
118
1678-1688
2017
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2
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1
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2
2
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743036
Posset
Understanding cerebral L-lysi ...
Mus musculus
J. Inherit. Metab. Dis.
38
265-272
2015
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1
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1
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3
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1
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1
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656324
Yoshida
Characterization of two fructo ...
Schizosaccharomyces pombe
J. Biosci. Bioeng.
97
278-280
2004
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1
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2
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2
1
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1
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1
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1
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2
1
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1
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4
1
1
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1
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392467
Naranjo
Conversion of pipecolic acid i ...
Penicillium chrysogenum
J. Bacteriol.
183
7165-7172
2001
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1
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1
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7
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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392469
Dodt
The human L-pipecolic acid oxi ...
Homo sapiens
Cell Biochem. Biophys.
32
313-316
2000
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1
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1
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1
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2
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1
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1
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1
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1
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1
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2
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1
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392470
Ijlst
Molecular cloning and expressi ...
Homo sapiens
Biochem. Biophys. Res. Commun.
270
1101-1105
2000
-
1
1
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-
-
-
-
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1
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4
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1
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1
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1
1
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1
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1
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1
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392471
Zabriskie
-
Mechanism-based inactivity of ...
Macaca mulatta
Bioorg. Med. Chem. Lett.
7
457-462
1997
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2
1
1
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1
1
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1
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1
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1
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392472
Zabriskie
-
Stereochemical course of the o ...
Macaca mulatta
J. Am. Chem. Soc.
119
6446-6447
1997
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1
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1
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1
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1
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2
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392473
Rao
L-Pipecolic acid oxidation in ...
Rattus norvegicus
Biochim. Biophys. Acta
1164
29-35
1993
2
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2
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2
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1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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392475
Rao
L-Pipecolic acid metabolism in ...
Homo sapiens
Biochim. Biophys. Acta
1038
295-299
1990
-
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2
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1
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2
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2
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1
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1
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1
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392476
Wanders
L-pipecolate oxidase: a distin ...
Homo sapiens
Biochem. Biophys. Res. Commun.
164
550-555
1989
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1
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2
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1
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2
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1
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1
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1
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392477
Mihalik
L-Pipecolic acid oxidation in ...
Macacine alphaherpesvirus 1, Oryctolagus cuniculus
J. Biol. Chem.
264
2509-2517
1989
1
2
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4
2
2
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2
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2
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5
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4
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1
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1
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4
1
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2
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2
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5
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4
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1
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392478
Kinzel
Lysine biosynthesis in Rhodoto ...
Rhodotorula glutinis
J. Bacteriol.
151
1073-1077
1982
-
-
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5
1
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1
1
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1
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2
2
1
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2
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1
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1
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5
1
1
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1
1
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2
1
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2
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1
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