Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 3.5 | L-proline | pH 8.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe | |
4.07 | - |
L-pipecolate | pH 8.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, about, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
98000 | - |
recombinant enzyme, gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | enzyme is involved in L-lysine metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
gene FAP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM109 | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-pipecolate + O2 | highly specific for | Schizosaccharomyces pombe | 2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2 | - |
? | |
L-proline + O2 | low activity | Schizosaccharomyces pombe | ? | - |
? | |
additional information | enzyme is involved in L-lysine metabolism | Schizosaccharomyces pombe | ? | - |
? | |
additional information | no activity with fructosyl-amino acids, L-saccharopine, and sarcosine | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, about, recombinant enzyme, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Fap1 | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently attached | Schizosaccharomyces pombe |