BRENDA - Enzyme Database show
show all sequences of 1.5.4.1

The clot gene of Drosophila melanogaster encodes a conserved member of the thioredoxin-like protein superfamily

Giordano, E.; Peluso, I.; Rendina, R.; Digilio, A.; Furia, M.; Mol. Genet. Genomics 268, 692-697 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
required for the in vitro assay
Drosophila melanogaster
Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
Drosophila melanogaster
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
pyrimidodiazepine + glutathione disulfide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
Q9VMQ9
gene clot
-
Reaction
Reaction
Commentary
Organism
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
active site contains the sequence Cys-Pro-Tyr-Cys
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
-
656799
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
6-pyruvoyl-tetrahydropterin + glutathione
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
656799
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
required for the in vitro assay
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
Drosophila melanogaster
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
pyrimidodiazepine + glutathione disulfide
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-pyruvoyl-tetrahydropterin + glutathione
-
656799
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
6-pyruvoyl-tetrahydropterin + glutathione
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
656799
Drosophila melanogaster
pyrimidodiazepine + glutathione disulfide
-
-
-
?
Other publictions for EC 1.5.4.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
671875
Kim
Identification and characteris ...
Drosophila melanogaster
Biochem. J.
398
451-460
2006
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1
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656799
Giordano
The clot gene of Drosophila me ...
Drosophila melanogaster
Mol. Genet. Genomics
268
692-697
2003
1
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437751
Wiederrecht
Purification and properties of ...
Drosophila melanogaster
J. Biol. Chem.
259
14121-14127
1984
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2
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1
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